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Eif4e3 (NM_025829) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202188
Eif4e3 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4E member 3 (Eif4e3)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Eif4e3 or NM_025829"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eif4e3
Synonyms 1300018P11Rik; AI451927; eIF4E-3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202188 representing NM_025829
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGCCCCCGGCTGCCGCACCCCCCGGGGCTAACGAGCCTCTCGACAAGGCTCTGTCCGCGTTGC
CGCCGGAGCCGGGCGGTGTCCCGCTGCACTCGCCCTGGACCTTCTGGCTGGACAGATCACTCCCTGGTGC
CACAGCAGCCGAGTGTGCCTCGAATCTGAAGAAAATCTACACGGTACAAACAGTGCAGATATTCTGGAGT
GTATACAATAACATCCCTCCTGTGACCAGCCTGCCTTTGAGATGTAGTTATCACTTAATGAGAGGAGAGA
GGCGACCACTTTGGGAGGAGGAGAGTAATGCGAAGGGTGGCGTGTGGAAGATGAAAGTTCCCAAGGACAG
CACGTCCACAGTTTGGAAAGAATTGTTGTTAGCGACCATTGGAGAGCAGTTCACAGACTGTGCTGCAGCA
GATGACGAGATCATCGGAGTCAGCGTGAGTGTGCGGGACCGAGAGGATGTCGTCCAGGTGTGGAATGTCA
ACGCCTCACTAGTGGGCGAAGCGACTGTGCTAGAGAAGATCCACCAGCTCCTGCCCCACATAGCTTTTAA
AGCAGTGTTTTATAAACCCCATGAAGAGCATCATGCTTTTGAAGGTGGACGTGGAAAACAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202188 representing NM_025829
Red=Cloning site Green=Tags(s)

MALPPAAAPPGANEPLDKALSALPPEPGGVPLHSPWTFWLDRSLPGATAAECASNLKKIYTVQTVQIFWS
VYNNIPPVTSLPLRCSYHLMRGERRPLWEEESNAKGGVWKMKVPKDSTSTVWKELLLATIGEQFTDCAAA
DDEIIGVSVSVRDREDVVQVWNVNASLVGEATVLEKIHQLLPHIAFKAVFYKPHEEHHAFEGGRGKH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025829
ORF Size 621 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025829.4
RefSeq Size 2514 bp
RefSeq ORF 624 bp
Locus ID 66892
UniProt ID Q9DBB5
Cytogenetics 6 D3
Summary Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. May act as an inhibitor of EIF4E1 activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025829" in other vectors (4)
SKU Description Size Price
MC201546 Eif4e3 (untagged) - Mouse eukaryotic translation initiation factor 4E member 3 (Eif4e3), (10ug) 10 ug
$300.00
MR202188 Eif4e3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E member 3 (Eif4e3) 10 ug
$289.00 $300.00
MR202188L3 Lenti ORF clone of Eif4e3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4E member 3 (Eif4e3) 10 ug
$600.00
MR202188L4 Lenti ORF clone of Eif4e3 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4E member 3 (Eif4e3) 10 ug
$600.00

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