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Ict1 (BC028523) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202185
Ict1 (tGFP-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Ict1 or BC028523"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ict1
Synonyms 1110001A02Rik; 1110002E03Rik; MRP-L58
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202185 representing BC028523
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCGCCTGGGGCCTACGCTGGGGCCTGAGCCGAACTGGAACGTTGTTGCTCGCGCCGCCCGCGC
GCTGTGCTCGCCGGGCTCTGCACAGACAGGTAGACGGCACCACGTTCCAGAGCATCTACAGCCTGGACAA
GCTGTACCCCGAGTCCAAGGGCGCGGATACCGCCTGGAAGGTCCCGGAGCATGCAAAACAAGCCAGCAGT
TACATCCCTCTGGATCGGTTAAGCATATCTTACTGTCGGAGCAGCGGTCCTGGTGGGCAGAATGTGAACA
AAGTGAATTCCAAGGCTGAAGTAAGGTTTCACTTGGCCAGTGCAGACTGGATTGAGGAGCCCGTGCGCCA
GAAGATTGCCCTCACGCATAAAAATAAGATCAACAAGGCAGGAGAGCTGGTACTTACCTCTGAGAGCAGC
CGGTATCAGTTCCGCAATCTGGCAGAATGCCTACAGAAAATTCGAGACATGATTGCCGAGGCCAGCCAGG
TACCCAAAGAGCCATCCAAGGAAGATGCTCGGCTTCAGAGACTCAGGATTGAAAAGATGAATCGGGAAAG
GCTACGACAGAAAAGACTAAACTCTGCCCTAAAGACCAGCAGGAGGATGACTATGGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202185 representing BC028523
Red=Cloning site Green=Tags(s)

MATAWGLRWGLSRTGTLLLAPPARCARRALHRQVDGTTFQSIYSLDKLYPESKGADTAWKVPEHAKQASS
YIPLDRLSISYCRSSGPGGQNVNKVNSKAEVRFHLASADWIEEPVRQKIALTHKNKINKAGELVLTSESS
RYQFRNLAECLQKIRDMIAEASQVPKEPSKEDARLQRLRIEKMNRERLRQKRLNSALKTSRRMTMD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC028523
ORF Size 618 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC028523, AAH28523
RefSeq Size 879 bp
RefSeq ORF 620 bp
Locus ID 68572
Cytogenetics 11 E2
Summary Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit (PubMed:20869366). Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC028523" in other vectors (4)
SKU Description Size Price
MC206924 Ict1 (untagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122), (10ug) 10 ug
$300.00
MR202185 Ict1 (Myc-DDK-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122) 10 ug
$289.00 $300.00
MR202185L3 Lenti ORF clone of Ict1 (Myc-DDK-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122) 10 ug
$600.00
MR202185L4 Lenti ORF clone of Ict1 (mGFP-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.