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Ict1 (BC028523) Mouse Untagged Clone

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SKU
MC206924
Ict1 (untagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $300.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ict1
Synonyms 1110001A02Rik; 1110002E03Rik; MRP-L58
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC206924 representing BC028523.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGACCGCCTGGGGCCTACGCTGGGGCCTGAGCCGAACTGGAACGTTGTTGCTCGCGCCGCCCGCG
CGCTGTGCTCGCCGGGCTCTGCACAGACAGGTAGACGGCACCACGTTCCAGAGCATCTACAGCCTGGAC
AAGCTGTACCCCGAGTCCAAGGGCGCGGATACCGCCTGGAAGGTCCCGGAGCATGCAAAACAAGCCAGC
AGTTACATCCCTCTGGATCGGTTAAGCATATCTTACTGTCGGAGCAGCGGTCCTGGTGGGCAGAATGTG
AACAAAGTGAATTCCAAGGCTGAAGTAAGGTTTCACTTGGCCAGTGCAGACTGGATTGAGGAGCCCGTG
CGCCAGAAGATTGCCCTCACGCATAAAAATAAGATCAACAAGGCAGGAGAGCTGGTACTTACCTCTGAG
AGCAGCCGGTATCAGTTCCGCAATCTGGCAGAATGCCTACAGAAAATTCGAGACATGATTGCCGAGGCC
AGCCAGGTACCCAAAGAGCCATCCAAGGAAGATGCTCGGCTTCAGAGACTCAGGATTGAAAAGATGAAT
CGGGAAAGGCTACGACAGAAAAGACTAAACTCTGCCCTAAAGACCAGCAGGAGGATGACTATGGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN BC028523
Insert Size 621 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC028523
RefSeq Size 879 bp
RefSeq ORF 620 bp
Locus ID 68572
Cytogenetics 11 E2
MW 23.5 kDa
Summary Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit (PubMed:20869366). Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes (By similarity).UniProtKB/Swiss-Prot Function
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"BC028523" in other vectors (4)
SKU Description Size Price
MG202185 Ict1 (tGFP-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122) 10 ug
$500.00
MR202185 Ict1 (Myc-DDK-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122) 10 ug
$289.00 $300.00
MR202185L3 Lenti ORF clone of Ict1 (Myc-DDK-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122) 10 ug
$600.00
MR202185L4 Lenti ORF clone of Ict1 (mGFP-tagged) - Mouse immature colon carcinoma transcript 1 (cDNA clone MGC:41300 IMAGE:2938122) 10 ug
$600.00

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