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Rflna (NM_028443) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202111
Fam101a (tGFP-tagged) - Mouse RIKEN cDNA 3110032G18 gene (3110032G18Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rflna
Synonyms 3110032G18Rik; cfm; cfm2; Fam101a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202111 representing NM_028443
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGTCACCTGCATCTGCAGGCCATGGGGGACACGCGGGAGCAGAGCCGCGACGGCTTGCTGGACA
GTCCCGATTCAGGACTGCCCCCCAGTCCCAGTCCTAGCCCGCCCTTCTACGCCCTGTCGCCAGGCACCCT
GGACACCCGGACCACCACTGAAGCTCCAGCGGCTCCTTCCCTCTTCCAAACTCCCCCAGCTCTGGAGATG
AGGTCACGGTTGCTGCCTGTGTTCTTTGGGGAGAGCATCGAGGTGGACCCAGAACCCGCACATGAAATCC
GCTGCAACTCTGAGATCACCTACGCCTCAGAGAGATACTTCCGAGACAAGATCTTCTACGCTCCAGTGCC
CACGGTCACGGCCTACAGTGAGACCATAGTGGCGGCGCCCAACTGCACCTGGCGGAGCTACCGCAGCCAG
CTGACCCTGGAGCCTCGCCCACGGGCCCTGCGGTTCGGGAGCACCGCCATCATCTTCCCCAAACTGGCCC
GCAGCTCCTTCCGCACCACCCTGCACTGCAGCCTCGGCCAGCCTCGGCACTGGTACTCCTCCAGCCTGCA
GCTGCGGCGGTGCGGGGACCCTACGCCCGGTCCCAGCTGTCCTGATGTACTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202111 representing NM_028443
Red=Cloning site Green=Tags(s)

MVGHLHLQAMGDTREQSRDGLLDSPDSGLPPSPSPSPPFYALSPGTLDTRTTTEAPAAPSLFQTPPALEM
RSRLLPVFFGESIEVDPEPAHEIRCNSEITYASERYFRDKIFYAPVPTVTAYSETIVAAPNCTWRSYRSQ
LTLEPRPRALRFGSTAIIFPKLARSSFRTTLHCSLGQPRHWYSSSLQLRRCGDPTPGPSCPDVL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028443
ORF Size 612 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028443.3
RefSeq Size 1668 bp
RefSeq ORF 615 bp
Locus ID 73121
UniProt ID Q7TS73
Cytogenetics 5 G1.1
Summary Involved in the regulation of the perinuclear actin network and nuclear shape through interaction with filamins. Plays an essential role in the formation of cartilaginous skeletal elements.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028443" in other vectors (4)
SKU Description Size Price
MC211384 Fam101a (untagged) - Mouse family with sequence similarity 101, member A (Fam101a), (10ug) 10 ug
$330.00
MR202111 Fam101a (Myc-DDK-tagged) - Mouse family with sequence similarity 101, member A (Fam101a) 10 ug
$289.00 $300.00
MR202111L3 Lenti ORF clone of Fam101a (Myc-DDK-tagged) - Mouse family with sequence similarity 101, member A (Fam101a) 10 ug
$600.00
MR202111L4 Lenti ORF clone of Fam101a (mGFP-tagged) - Mouse family with sequence similarity 101, member A (Fam101a) 10 ug
$600.00

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