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Rab30 (NM_029494) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202099
Rab30 (tGFP-tagged) - Mouse RAB30, member RAS oncogene family (Rab30)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rab30
Synonyms 5033421K01Rik; AI323892; Rsb30
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202099 representing NM_029494
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTATGGAAGATTATGATTTCCTGTTCAAAATTGTTTTAATTGGCAACGCTGGAGTGGGAAAGACGT
GCCTAGTCCGAAGATTCACTCAGGGTCTTTTCCCCCCAGGTCAAGGAGCCACAATTGGAGTTGATTTTAT
GATTAAGACAGTGGAGATTAATGGTGAAAAAGTGAAGTTACAGATCTGGGACACCGCAGGTCAAGAGAGA
TTTCGCTCCATCACTCAAAGTTACTATCGAAGCGCCAATGCCTTGATCCTTACCTATGACATCACCTGTG
AGGAATCCTTCCGCTGCCTTCCTGAGTGGTTGCGGGAGATAGAACAGTATGCTAGCAATAAAGTCATCAC
TGTGTTAGTAGGCAACAAGATTGACCTGGCTGAAAGGCGAGAGGTCTCCCAGCAGAGAGCAGAAGAGTTC
TCAGAGGCTCAGGACATGTATTACCTGGAGACTTCAGCCAAGGAATCCGACAATGTGGAGAAACTCTTCC
TTGACTTAGCATGCCGACTCATCAGCGAAGCAAGACAGAACACACTGGTGAACAATGTATCGTCACCCTT
ACCCGGAGAGGGGAAAAGCATCAGCTATTTGACTTGTTGTAATTTCAAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202099 representing NM_029494
Red=Cloning site Green=Tags(s)

MSMEDYDFLFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEINGEKVKLQIWDTAGQER
FRSITQSYYRSANALILTYDITCEESFRCLPEWLREIEQYASNKVITVLVGNKIDLAERREVSQQRAEEF
SEAQDMYYLETSAKESDNVEKLFLDLACRLISEARQNTLVNNVSSPLPGEGKSISYLTCCNFN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029494
ORF Size 609 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029494.2, NP_083770.2
RefSeq Size 2007 bp
RefSeq ORF 612 bp
Locus ID 75985
UniProt ID Q923S9
Cytogenetics 7 E1
Summary The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). Required for maintaining the structural integrity of the Golgi apparatus, possibly by mediating interactions with cytoplasmic scaffolding proteins (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029494" in other vectors (4)
SKU Description Size Price
MC203956 Rab30 (untagged) - Mouse RAB30, member RAS oncogene family (Rab30), (10ug) 10 ug
$300.00
MR202099 Rab30 (Myc-DDK-tagged) - Mouse RAB30, member RAS oncogene family (Rab30) 10 ug
$289.00 $300.00
MR202099L3 Lenti ORF clone of Rab30 (Myc-DDK-tagged) - Mouse RAB30, member RAS oncogene family (Rab30) 10 ug
$600.00
MR202099L4 Lenti ORF clone of Rab30 (mGFP-tagged) - Mouse RAB30, member RAS oncogene family (Rab30) 10 ug
$600.00

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