Commd10 (NM_178377) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG202092
Commd10 (tGFP-tagged) - Mouse COMM domain containing 10 (Commd10)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Commd10
Synonyms 2310003A05Rik; AU019438; DRWMS2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202092 representing NM_178377
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGTCCGCAGCGCTGATCCTGCCCGAGAGCCCCAGCATGAAGAAAGCGGTGCCGCTGATAAATG
CAATAGACACGGGGAGATTCCCACGACTTCTCAGCCGGATTCTTCAAAAGCTCCACCTGAAGGCCGAGAG
CAGTTTCAGTGAAGAAGAGGAAGAAAAACTTCAGGCTGCATTCTCTCTAGAGAAACAAGAACTTCACCTG
GTTCTGGAGACAATATCATTTGTTTTGGAACAGGCAGTGTATCACAACGTGAAGCCAGCAGCACTGCAGC
AGCAACTAGAGATGATTCATCTTAGGAAAGACAAAGCGGAAGCCTTTGCCAGTGCTTGGTCGGCTATGGG
TCAGGAGACAGTTGAGAAGTTCCGCCAGAGAATCCTGGGCCCTCACAAGCTAGAGACAGTGGGATGGCAG
CTTAACCTGCAAATGGCCCACTCTGCTCAAGCAAAGCTGCAGTCTCCCCAGGCCGTGCTGCAACTGGGAG
TGAGCAAGGAAGACGCCAAGAACGTGGAGAAAGTTCTCGTGGAATTCAATCACAAGGAGCTGTTCGATTT
CTACAACAAGCTGGAGACCATACAAGCGCAGCTGGACTCCCTCACA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202092 representing NM_178377
Red=Cloning site Green=Tags(s)

MAASAALILPESPSMKKAVPLINAIDTGRFPRLLSRILQKLHLKAESSFSEEEEEKLQAAFSLEKQELHL
VLETISFVLEQAVYHNVKPAALQQQLEMIHLRKDKAEAFASAWSAMGQETVEKFRQRILGPHKLETVGWQ
LNLQMAHSAQAKLQSPQAVLQLGVSKEDAKNVEKVLVEFNHKELFDFYNKLETIQAQLDSLT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178377
ORF Size 606 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178377.4, NP_848464.1
RefSeq Size 1550 bp
RefSeq ORF 609 bp
Locus ID 69456
UniProt ID Q8JZY2
Cytogenetics 18 C
Summary May modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes. May down-regulate activation of NF-kappa-B.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178377" in other vectors (4)
SKU Description Size Price
MC207683 Commd10 (untagged) - Mouse COMM domain containing 10 (Commd10), (10ug) 10 ug
$330.00
MR202092 Commd10 (Myc-DDK-tagged) - Mouse COMM domain containing 10 (Commd10) 10 ug
$289.00 $300.00
MR202092L3 Lenti ORF clone of Commd10 (Myc-DDK-tagged) - Mouse COMM domain containing 10 (Commd10) 10 ug
$600.00
MR202092L4 Lenti ORF clone of Commd10 (mGFP-tagged) - Mouse COMM domain containing 10 (Commd10) 10 ug
$600.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.