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Guca1b (NM_146079) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202065
Guca1b (tGFP-tagged) - Mouse guanylate cyclase activator 1B (Guca1b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Guca1b
Synonyms BC018258; GCAP2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202065 representing NM_146079
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGCAGCTGAGCTGGGAAGAGGCGGAGGCGGCCGGGGAGATGGATGTGGCGGAGTTGCAGGAGT
GGTACAAGAAGTTTGTCGTGGAGTGTCCCAGTGGGACGCTCTTCATGCACGAGTTCAAGCGCTTCTTCAA
GGTCACGGGCAATGAGGAGGCCAGCCAGTATGTAGAGAGCATGTTCCGAGCCTTCGACAAGAATGGGGAC
AACACCATTGACTTCCTGGAGTATGTGGCAGCCCTGAACCTCGTGCTGAGGGGCAGCCTGGAACACAAGC
TCAAGTGGACCTTCAAGATCTATGACAAGGACCGAAATGGCTGCATCGACCGCCTGGAACTCCTGGACAT
TGTGGAGGCGATTTACAAGCTGAAGAAAGCCTGCCGAGCGGAGCTGGATCTGGAACACCAGGGCCAGCTG
CTCACGCCCGAGGAGGTGGTGGACAGGATCTTCCTCCTGGTGGACGAGAATGGAGACGGCCAGCTGTCTC
TGACCGAGTTCATCGAGGGCGCCCGTCGCGATAAGTGGGTAATGAAGATGCTACAAATGGATATCAACCC
AGGCTGCTGGATCACGCAGCAGAGGCGGAGAAGCGCCATGTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202065 representing NM_146079
Red=Cloning site Green=Tags(s)

MGQQLSWEEAEAAGEMDVAELQEWYKKFVVECPSGTLFMHEFKRFFKVTGNEEASQYVESMFRAFDKNGD
NTIDFLEYVAALNLVLRGSLEHKLKWTFKIYDKDRNGCIDRLELLDIVEAIYKLKKACRAELDLEHQGQL
LTPEEVVDRIFLLVDENGDGQLSLTEFIEGARRDKWVMKMLQMDINPGCWITQQRRRSAMF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146079
ORF Size 603 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146079.1, NP_666191.1
RefSeq Size 1681 bp
RefSeq ORF 606 bp
Locus ID 107477
UniProt ID Q8VBV8
Cytogenetics 17 C
Summary Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_146079" in other vectors (4)
SKU Description Size Price
MC201675 Guca1b (untagged) - Mouse guanylate cyclase activator 1B (Guca1b), (10ug) 10 ug
$300.00
MR202065 Guca1b (Myc-DDK-tagged) - Mouse guanylate cyclase activator 1B (Guca1b) 10 ug
$289.00 $300.00
MR202065L3 Lenti ORF clone of Guca1b (Myc-DDK-tagged) - Mouse guanylate cyclase activator 1B (Guca1b) 10 ug
$600.00
MR202065L4 Lenti ORF clone of Guca1b (mGFP-tagged) - Mouse guanylate cyclase activator 1B (Guca1b) 10 ug
$600.00

Plasmid Map for MG202065

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG202065
Circular map for MG202065

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