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Mrpl12 (NM_027204) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202047
Mrpl12 (tGFP-tagged) - Mouse mitochondrial ribosomal protein L12 (Mrpl12)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Mrpl12 or NM_027204"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrpl12
Synonyms 0610034O11Rik; 1500031N16Rik; MRP-L12; Rpml12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202047 representing NM_027204
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCCGGTGGCGGCTAGCCGCTGCCTGTGGGGGCCACGGCTTGGACTCCGGGGGGCAGCGCTTCGCC
TTGCCAGGCAACAGATGCCCAGCGTCTGTGCAGCGCGGCAGTTGAGAAGCAGCAGCCACCGAAGGAGTGA
AGCACTCGCCGGCGCACCTCTGGATAATGCTCCCAAAGAATACCCCCCCAAGATCCAGCAGCTGGTCCAA
GACATTGCCAGCCTTACGCTCCTGGAGATCTCAGACCTCAACGAACTCCTGAAGAAAACATTGAAGATCC
AGGATGTCGGCCTTATGCCAATGGGTGGCATGGTGCCTGGTCCCGTCTCTGCTGCAGCACCGGCCTCTGA
GGCGGCCGAGGAGGAAGACGTCCCCAAACAGAAAGAGCGGACACACTTCACCGTCCGCCTGACAGAGGCA
AAGCCTGTGGACAAAGTAAAGCTGATCAAGGAGATCAAGAACTACGTCCAGGGCATCAACCTCGTCCAGG
CCAAGAAGCTGGTAGAGTCCCTGCCCCAGGAAATCAAAGCCAACGTCGCCAAAGCTGAGGCCGAGAAGAT
CAAAGCAGCCTTGGAGGCAGTGGGTGGCACCGTGGTTCTGGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202047 representing NM_027204
Red=Cloning site Green=Tags(s)

MLPVAASRCLWGPRLGLRGAALRLARQQMPSVCAARQLRSSSHRRSEALAGAPLDNAPKEYPPKIQQLVQ
DIASLTLLEISDLNELLKKTLKIQDVGLMPMGGMVPGPVSAAAPASEAAEEEDVPKQKERTHFTVRLTEA
KPVDKVKLIKEIKNYVQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027204
ORF Size 603 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027204.3
RefSeq Size 963 bp
RefSeq ORF 606 bp
Locus ID 56282
UniProt ID Q9DB15
Cytogenetics 11 E2
Summary As a component of the mitochondrial large ribosomal subunit, it plays a role in mitochondrial translation. Associates with mitochondrial RNA polymerase to activate transcription.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027204" in other vectors (4)
SKU Description Size Price
MC200407 Mrpl12 (untagged) - Mouse mitochondrial ribosomal protein L12 (Mrpl12), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MR202047 Mrpl12 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L12 (Mrpl12), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $300.00
MR202047L3 Lenti ORF clone of Mrpl12 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L12 (Mrpl12), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR202047L4 Lenti ORF clone of Mrpl12 (mGFP-tagged) - Mouse mitochondrial ribosomal protein L12 (Mrpl12), nuclear gene encoding mitochondrial protein 10 ug
$600.00

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