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Nt5c (NM_015807) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202028
Nt5c (tGFP-tagged) - Mouse 5',3'-nucleotidase, cytosolic (Nt5c)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Nt5c or NM_015807"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nt5c
Synonyms Dnt; Dnt1; Umph-2; Umph2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202028 representing NM_015807
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGAAGCGGCCCGTGCGCGTGCTGGTGGACATGGACGGCGTGCTAGCTGACTTCGAGTCCGGCC
TCCTGCAGGGTTTTCGCCGCCGCTTTCCCGAGGAACCGCACGTGCCGCTGGAACAGCGCCGGGGCTTCCT
CGCCAACGAGCAGTACGGAGCCCTGAGGCCGGACCTGGCGGAAAAAGTGGCCAGTGTGTATGAATCTCCA
GGCTTTTTCCTAAACTTGGAGCCCATCCCTGGGGCCTTGGACGCTTTGCGAGAGATGAACGACATGAAAG
ACACCGAGGTCTTCATCTGCACCACCCCACTGCTGAAGTACGACCACTGTGTGGGCGAGAAGTACCGCTG
GGTGGAGCAGAACCTGGGGCCAGAGTTTGTGGAGCGGATTATCTTGACTAGGGACAAGACGGTGGTCATG
GGAGACCTGCTCATTGATGACAAAGACAACATTCAAGGCCTAGAGGAGACCCCAAGCTGGGAGCACATCT
TGTTCACCTGCTGCCACAATCAGCACCTGGCCCTGCCCCCCACCAGGAGACGGCTGCTTTCCTGGAGTGA
CAACTGGAGGGGGATCATAGAGAGCAAGCGAGCCAGCCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202028 representing NM_015807
Red=Cloning site Green=Tags(s)

MAVKRPVRVLVDMDGVLADFESGLLQGFRRRFPEEPHVPLEQRRGFLANEQYGALRPDLAEKVASVYESP
GFFLNLEPIPGALDALREMNDMKDTEVFICTTPLLKYDHCVGEKYRWVEQNLGPEFVERIILTRDKTVVM
GDLLIDDKDNIQGLEETPSWEHILFTCCHNQHLALPPTRRRLLSWSDNWRGIIESKRASL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015807
ORF Size 600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015807.1, NP_056622.1
RefSeq Size 820 bp
RefSeq ORF 603 bp
Locus ID 50773
UniProt ID Q9JM14
Cytogenetics 11 E2
Summary Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_015807" in other vectors (4)
SKU Description Size Price
MC204174 Nt5c (untagged) - Mouse 5',3'-nucleotidase, cytosolic (Nt5c), (10ug) 10 ug
$300.00
MR202028 Nt5c (Myc-DDK-tagged) - Mouse 5',3'-nucleotidase, cytosolic (Nt5c) 10 ug
$289.00 $300.00
MR202028L3 Lenti ORF clone of Nt5c (Myc-DDK-tagged) - Mouse 5',3'-nucleotidase, cytosolic (Nt5c) 10 ug
$600.00
MR202028L4 Lenti ORF clone of Nt5c (mGFP-tagged) - Mouse 5',3'-nucleotidase, cytosolic (Nt5c) 10 ug
$600.00

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