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Sssca1 (NM_020491) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG202014
Sssca1 (tGFP-tagged) - Mouse Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) (Sssca1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sssca1
Synonyms 1500016H19Rik; C184L
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG202014 representing NM_020491
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTGAACGGTGCAGATGTCGACGATTTCACCTGGGAGCCTCCGACTGAAGCTGAGACGAAGGTGC
TGCAGGCGCGACGGGAGCGGCAGGATCGCATCTCCCGTCTGATGGGGGACTACCTGCTCCGTGGTTACCG
CATGCTGGGCGACACGTGCGCCGACTGCGGGACGATCCTCCTGCAAGATAAACAGCGGAAAATCTACTGT
GTGGCTTGTCAGGAGCTCGACTCAGACGTGGATAAAGATAACCCGGCACTGAATGCACAGGCGGCGCTCT
CTCAAGCTCGGGAACACCAGCTGGCCTCATCTACAGAGCCTGCCTCGAGCTCTCGGCCCCCATCCCAGCC
ACCAGTACCCCGTCCAGAGCACTGTGAAGGCGCAGCAGCGGGGCTCAAGGCAGCCCAGGCGCCTCCTCTT
CCGGCTGCACCTCCAAATACGGACGCGGTGGCTTCCACACAGACGGCCCTCCTGCAGAAGCTAACCTGGG
CGTCAGTCGAGCTGGGCTCTAGCACCTCCTTGGAGACTAGTATCCAGCTCTGTGGGCTTATCCGGGCTTG
CGCCGAGGCCCTAGGCAGCCTGAAACAGCTGGATCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG202014 representing NM_020491
Red=Cloning site Green=Tags(s)

MALNGADVDDFTWEPPTEAETKVLQARRERQDRISRLMGDYLLRGYRMLGDTCADCGTILLQDKQRKIYC
VACQELDSDVDKDNPALNAQAALSQAREHQLASSTEPASSSRPPSQPPVPRPEHCEGAAAGLKAAQAPPL
PAAPPNTDAVASTQTALLQKLTWASVELGSSTSLETSIQLCGLIRACAEALGSLKQLDH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020491
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020491.4, NP_065237.3
RefSeq Size 690 bp
RefSeq ORF 600 bp
Locus ID 56390
UniProt ID P56873
Cytogenetics 19 A
Summary Might play a role in mitosis. Could be a centromere-associated protein. Antigenic molecule. May induce anti-centromere antibodies.UniProtKB/Swiss-Prot Function
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"NM_020491" in other vectors (4)
SKU Description Size Price
MC201963 Sssca1 (untagged) - Mouse Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) (Sssca1), (10ug) 10 ug
$300.00
MR202014 Sssca1 (Myc-DDK-tagged) - Mouse Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) (Sssca1) 10 ug
$289.00 $300.00
MR202014L3 Lenti ORF clone of Sssca1 (Myc-DDK-tagged) - Mouse Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) (Sssca1) 10 ug
$600.00
MR202014L4 Lenti ORF clone of Sssca1 (mGFP-tagged) - Mouse Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) (Sssca1) 10 ug
$600.00

Plasmid Map for MG202014

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG202014
Circular map for MG202014

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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