Rnf41 (BC019415) Mouse Tagged ORF Clone

SKU MG201892

Rnf41 (tGFP-tagged) - Mouse ring finger protein 41 (cDNA clone MGC:30902 IMAGE:4014525)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Rnf41
• Synonyms: 4933415P08Rik, FLRF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG201892 representing BC019415; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCCAAAGATGAACTGCCAAACCACAATTGCATTAAGCACCTGCGCTCCGTGGTCCAGCAGCAGCAGT; CGCGCATCGCAGAGCTGGAGAAGACCAGGGCTGAACACAAGCACCAGCTGGCAGAGCAGAAGCGAGACAT; TCAGCTGCTGAAGGCGTATATGCGAGCCATCCGCAGTGTCAACCCCAACCTTCAGAACCTGGAGGAGACA; ATCGAATACAACGAGATCCTCGAGTGGGTGAACTCCCTGCAGCCGGCAAGGGTGACCCGCTGGGGGGGCA; TGATCTCCACTCCTGATGCTGTGCTCCAGGCTGTCATCAAGCGCTCCCTCGTGGAAAGTGGCTGCCCGGC; CTCCATCGTCAACGAGCTGATTGAAAATGCCCATGAACGCAGTTGGCCCCAGGGTCTGGCCACACTAGAG; ACAAGACAGATGAACCGGCGCTACTATGAGAACTACGTGGCCAAGCGCATCCCTGGCAAGCAGGCTGTAG; TGGTGATGGCCTGTGAGAACCAGCACATGGGGGACGACATGGTGCAGGAGCCAGGGCTCGTCATGATATT; TGCGCATGGTGTGGAGGAGATA; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG201892 representing BC019415; Red=Cloning site Green=Tags(s); ; MPKDELPNHNCIKHLRSVVQQQQSRIAELEKTRAEHKHQLAEQKRDIQLLKAYMRAIRSVNPNLQNLEET; IEYNEILEWVNSLQPARVTRWGGMISTPDAVLQAVIKRSLVESGCPASIVNELIENAHERSWPQGLATLE; TRQMNRRYYENYVAKRIPGKQAVVVMACENQHMGDDMVQEPGLVMIFAHGVEEI; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019415
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC019415, AAH19415
• RefSeq Size: 1739 bp
• RefSeq ORF: 584 bp
• Locus ID: 67588
• Cytogenetics: 10 76.55 cM
• Summary: Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88 (By similarity). Negatively regulates MYD88-dependent production of proinflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus. Promotes also activation of TBK1 and IRF3 (PubMed:19483718). Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (PubMed:18495327). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control (PubMed:24949970).[UniProtKB/Swiss-Prot Function]