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Rhoc (NM_007484) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201861
Rhoc (tGFP-tagged) - Mouse ras homolog gene family, member C (Rhoc)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Rhoc or NM_007484"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rhoc
Synonyms AI324259; Arh9; Arhc
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201861 representing NM_007484
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGATCCGAAAGAAGCTGGTGATTGTGGGTGACGGTGCCTGTGGGAAGACCTGCCTCCTCATTG
TCTTCAGCAAAGATCAGTTTCCAGAGGTCTACGTGCCTACCGTCTTTGAGAACTATATAGCCGACATCGA
AGTGGATGGCAAGCAGGTGGAGCTGGCTCTGTGGGACACAGCTGGGCAAGAAGACTACGATCGCCTGCGG
CCTCTCTCCTACCCGGACACTGACGTCATCCTCATGTGTTTCTCCATTGACAGCCCTGACAGTTTGGAAA
ACATTCCTGAGAAGTGGACCCCAGAGGTGAAGCACTTTTGTCCCAACGTGCCCATCATCCTAGTGGGGAA
TAAAAAGGACCTGAGGCAAGATGAGCATACCAGGAGAGAGCTGGCCAAGATGAAACAGGAGCCGGTTCGA
TCTGAGGAAGGCCGGGACATGGCAAACAGGATCAGTGCCTTTGGCTACCTCGAGTGCTCGGCCAAGACTA
AGGAAGGGGTGCGGGAGGTATTTGAGATGGCCACTCGGGCGGGCCTACAGGTCCGGAAGAATAAGCGCCG
GAGGGGCTGTCCCATTCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201861 representing NM_007484
Red=Cloning site Green=Tags(s)

MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLR
PLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVR
SEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007484
ORF Size 579 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007484.2
RefSeq Size 1036 bp
RefSeq ORF 582 bp
Locus ID 11853
UniProt ID Q62159
Cytogenetics 3 F2.2
Summary Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007484" in other vectors (3)
SKU Description Size Price
MR201861 Rhoc (Myc-DDK-tagged) - Mouse ras homolog gene family, member C (Rhoc) 10 ug
$289.00 $300.00
MR201861L3 Lenti ORF clone of Rhoc (Myc-DDK-tagged) - Mouse ras homolog gene family, member C (Rhoc) 10 ug
$600.00
MR201861L4 Lenti ORF clone of Rhoc (mGFP-tagged) - Mouse ras homolog gene family, member C (Rhoc) 10 ug
$600.00

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