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Pla2g12a (BC026812) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201855
Pla2g12a (tGFP-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pla2g12a or BC026812"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pla2g12a
Synonyms mGXII-1-PLA2, MGC58884, GXII, mGXII-1, Rossy
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201855 representing BC026812
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGACTCCGCGGCCCGCGCCCGCCCGGGGCCCCGCGCTCCTCCTCCTCCTGCTGCTGGCCACTGCGC
GCGGGCAGGAACAGGACCAGACCACCGACTGGAGGGCCACCCTCAAGACCATCCGCAACGGCATCCACAA
GATAGACACGTACCTCAACGCCGCGCTGGACCTGCTGGGCGGGGAGGACGGGCTCTGCCAGTACAAGTGC
AGCGACGGATCGAAGCCTGTTCCACGCTATGGATATAAACCATCTCCACCAAATGGCTGTGGCTCTCCAC
TGTTTGGCGTTCATCTGAACATAGGTATCCCTTCCCTGACCAAGTGCTGCAACCAGCACGACAGATGCTA
TGAGACCTGCGGGAAAAGCAAGAACGACTGTGACGAGGAGTTCCAGTACTGCCTCTCCAAGATCTGCAGA
GACGTGCAGAAGACGCTCGGACTATCTCAGAACGTCCAGGCATGTGAGACAACGGTGGAGCTCCTCTTTG
ACAGCGTCATCCATTTAGGCTGCAAGCCATACCTGGACAGCCAGCGGGCTGCATGCTGGTGTCGTTATGA
AGAAAAAACAGATCTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201855 representing BC026812
Red=Cloning site Green=Tags(s)

MVTPRPAPARGPALLLLLLLATARGQEQDQTTDWRATLKTIRNGIHKIDTYLNAALDLLGGEDGLCQYKC
SDGSKPVPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICR
DVQKTLGLSQNVQACETTVELLFDSVIHLGCKPYLDSQRAACWCRYEEKTDL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC026812
ORF Size 576 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC026812, AAH26812
RefSeq Size 1022 bp
RefSeq ORF 578 bp
Locus ID 66350
Cytogenetics 3 G3
Summary PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl-phosphatidylcholine or -phosphatidylethanolamine (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC026812" in other vectors (3)
SKU Description Size Price
MR201855 Pla2g12a (Myc-DDK-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431) 10 ug
$289.00 $300.00
MR201855L3 Lenti ORF clone of Pla2g12a (Myc-DDK-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431) 10 ug
$600.00
MR201855L4 Lenti ORF clone of Pla2g12a (mGFP-tagged) - Mouse phospholipase A2, group XIIA (cDNA clone MGC:25458 IMAGE:4456431) 10 ug
$600.00

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