Arl4c (NM_177305) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201837
Arl4c (tGFP-tagged) - Mouse ADP-ribosylation factor-like 4C (Arl4c)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arl4c
Synonyms A630084M22Rik; Arl7; LAK
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201837 representing NM_177305
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACATCTCCTCCAACATCTCGGCCTTCCAGTCTCTGCACATCGTTATGCTGGGCTTGGACTCGG
CCGGCAAGACTACGGTGCTCTACCGGCTCAAGTTCAACGAATTCGTGAACACTGTGCCCACTATCGGCTT
CAACACCGAGAAGATCAAACTGAGCAACGGCACTGCCAAGGGCATCAGCTGCCACTTCTGGGACGTGGGC
GGCCAGGAGAAGCTACGGCCGCTGTGGAAGTCCTACAGCCGCTGCACGGACGGCATCATCTACGTGGTGG
ATTCGGTGGACGTGGACCGGCTGGAGGAGGCCAAGACGGAGCTGCACAAGGTGACCAAGTTTGCGGAGAA
CCAGGGCACACCGCTCCTGGTTATCGCCAACAAGCAGGATCTGCCTAAGTCGCTGCCGGTGGCCGAGATT
GAGAAGCAACTGGCGCTGCACGAGCTCATCCCGGCCACCACCTACCATGTCCAGCCGGCGTGCGCCATCA
TCGGCGAGGGCCTCACCGAGGGCATGGACAAGCTCTATGAGATGATCCTGAAACGCAGGAAGTCTCTCAA
GCAGAAGAAGAAGCGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201837 representing NM_177305
Red=Cloning site Green=Tags(s)

MGNISSNISAFQSLHIVMLGLDSAGKTTVLYRLKFNEFVNTVPTIGFNTEKIKLSNGTAKGISCHFWDVG
GQEKLRPLWKSYSRCTDGIIYVVDSVDVDRLEEAKTELHKVTKFAENQGTPLLVIANKQDLPKSLPVAEI
EKQLALHELIPATTYHVQPACAIIGEGLTEGMDKLYEMILKRRKSLKQKKKR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177305
ORF Size 576 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177305.4
RefSeq Size 3966 bp
RefSeq ORF 579 bp
Locus ID 320982
UniProt ID P61208
Cytogenetics 1 D
Summary Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. May be involved in transport between a perinuclear compartment and the plasma membrane, apparently linked to the ABCA1-mediated cholesterol secretion pathway. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in the GDP-bound form. Regulates the microtubule-dependent intracellular vesicular transport from early endosome to recycling endosome process (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177305" in other vectors (4)
SKU Description Size Price
MC205099 Arl4c (untagged) - Mouse ADP-ribosylation factor-like 4C (Arl4c), (10ug) 10 ug
$300.00
MR201837 Arl4c (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 4C (Arl4c) 10 ug
$289.00 $300.00
MR201837L3 Lenti ORF clone of Arl4c (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 4C (Arl4c) 10 ug
$600.00
MR201837L4 Lenti ORF clone of Arl4c (mGFP-tagged) - Mouse ADP-ribosylation factor-like 4C (Arl4c) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.