Gins2 (NM_178856) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201730
Gins2 (tGFP-tagged) - Mouse RIKEN cDNA 4833427B12 gene (4833427B12Rik)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gins2
Synonyms 2210013I18Rik; 4833427B12Rik; AI323585; Pfs2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201730 representing NM_178856
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGCGGCCGAGGTGGAGTTTTTGGCCGAAAAGGAGTTGGTGACTATCATCCCGAACTTCAGTCTGG
ACAAGATCTACCTGATCGGGGGGGACCTGGGGCCCTTCAACCCCGGCTTACCCGTGGACGTGCCCCTGTG
GCTGGCCATTAACCTGAAACAGAGACAGAAGTGCCGCCTGTTACCTCCGGAATGGATGGATGTGGAGAAA
CTGGAACAGATGCGTGATGAGGAGCGGAAGGAGGAGACATTCACCCCAGTGCCCAGCCCGCACTACATGG
AGATCACGAAGCTCCTGCTGAATCATGCTTCTGACAACATCCCCAAAGCAGACACCATCCGGACACTGAT
CAAAGACCTGTGGGACACACGCATGGCCAAGCTTCGAGTGTCTGCTGACAGTTTTGTGCGGCAGCAAGAG
GCACATGCCAAGCTGGACAACCTGACCTTGATGGAGATCAGCAGCAGCGGGGCCTTCCTCACCCAGGCCC
TCAACCACATGTACAAGCTCCGCACAAACCTCCAGCCCTCCGAGAGCACCCAGTCGCAGGACTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201730 representing NM_178856
Red=Cloning site Green=Tags(s)

MDAAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVDVPLWLAINLKQRQKCRLLPPEWMDVEK
LEQMRDEERKEETFTPVPSPHYMEITKLLLNHASDNIPKADTIRTLIKDLWDTRMAKLRVSADSFVRQQE
AHAKLDNLTLMEISSSGAFLTQALNHMYKLRTNLQPSESTQSQDF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178856
ORF Size 555 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178856.1, NP_849187.1
RefSeq Size 1061 bp
RefSeq ORF 558 bp
Locus ID 272551
UniProt ID Q9D600
Cytogenetics 8 E1
Summary The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178856" in other vectors (4)
SKU Description Size Price
MC205256 Gins2 (untagged) - Mouse GINS complex subunit 2 (Psf2 homolog) (Gins2), (10ug) 10 ug
$300.00
MR201730 Gins2 (Myc-DDK-tagged) - Mouse GINS complex subunit 2 (Psf2 homolog) (Gins2) 10 ug
$289.00 $300.00
MR201730L3 Lenti ORF clone of Gins2 (Myc-DDK-tagged) - Mouse GINS complex subunit 2 (Psf2 homolog) (Gins2) 10 ug
$600.00
MR201730L4 Lenti ORF clone of Gins2 (mGFP-tagged) - Mouse GINS complex subunit 2 (Psf2 homolog) (Gins2) 10 ug
$600.00

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