Bloc1s5 (NM_139063) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201726
Bloc1s5 (tGFP-tagged) - Mouse muted (Muted)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bloc1s5
Synonyms 1810074A19Rik; mu; Muted
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201726 representing NM_139063
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGCGGAGGCACCGAGACCCCTGTAGCGTGCGACGCCGCCCAGGGCGGCAAGAAGCGGGACTCAC
TGGGGACTCCGGGTGCGGCGCACCTCATTATCAAGGATCTTGGAGAGATTCATTCCAGGCTGCTGGATCA
CAGACCAGTTACCCAAGGTGAAATCCGTTACTTTGTAAAAGAATTTGAAGAAAAACGTGGCCTTCGAGAA
TTGCGCGTTCTTAAGAACTTGGAGAATACGATCCAGGAAACAAATGAGTGCCTGCTTCCCAAATGCAGAG
AGACCATGGAGTGCGGCCTGGGGGAGACCCTGCAGAGATTGCAAGCAGCTAACGACTCCATCTGCAGACT
CCAGCAGAGAGAACAGGAACGGAAAAAGGTGATTAATGACTACTTGACAGCTAGTGAGAAGCGTCGTCTG
GTCCAGTGGGAGGAGTTCGTGAGCGGACAGCCGCAGCGCAGAGCTGAGGTGGACGAGGAGCACAGAAGAG
CCGTGGAGAGGCTCCGAGAGCAGTATGCAGCAATGGAGAAGGACCTGGCCAAGTTTTCCACCTTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201726 representing NM_139063
Red=Cloning site Green=Tags(s)

MSGGGTETPVACDAAQGGKKRDSLGTPGAAHLIIKDLGEIHSRLLDHRPVTQGEIRYFVKEFEEKRGLRE
LRVLKNLENTIQETNECLLPKCRETMECGLGETLQRLQAANDSICRLQQREQERKKVINDYLTASEKRRL
VQWEEFVSGQPQRRAEVDEEHRRAVERLREQYAAMEKDLAKFSTF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_139063
ORF Size 555 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139063.3
RefSeq Size 1791 bp
RefSeq ORF 558 bp
Locus ID 17828
UniProt ID Q8R015
Cytogenetics 13 18.35 cM
Summary Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_139063" in other vectors (4)
SKU Description Size Price
MC202173 Bloc1s5 (untagged) - Mouse muted (Muted), (10ug) 10 ug
$300.00
MR201726 Bloc1s5 (Myc-DDK-tagged) - Mouse muted (Muted) 10 ug
$289.00 $300.00
MR201726L3 Lenti ORF clone of Bloc1s5 (Myc-DDK-tagged) - Mouse muted (Muted) 10 ug
$600.00
MR201726L4 Lenti ORF clone of Bloc1s5 (mGFP-tagged) - Mouse muted (Muted) 10 ug
$600.00

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