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Tifa (NM_145133) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201692
Tifa (tGFP-tagged) - Mouse Traf2 binding protein (T2bp)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tifa or NM_145133"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tifa
Synonyms T2bp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201692 representing NM_145133
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACCTTTGAAGACGCTGATACAGAGGAGACGGTCACTTGTCTCCAGATGACCATTTACCATCCTG
GCCAACAAAGTGGGATATTTAAATCAATAAGGTTTTGCAGCAAAGAGAAGTTTCCTTCCATTGAAGTGGT
GAAATTTGGACGCAATTCCAACATGTGCCAGTATACGTTTCAGGACAAACAGGTGTCCCGAATTCAGTTT
GTTTTACAGCCGTTTAAACAGTTCAACAGCTCCGTTCTCTCGTTTGAAATAAAAAACATGAGCAAGAAAA
CCAGTTTGATGGTAGACAACCAGGAGCTCGGCTACCTCAATAAAATGGACCTGCCTTACAAGTGTATGCT
CAGGTTCGGAGAGTATCAGTTCCTGTTGCAGAAGGAAGACGGAGAGTCGGTGGAATCTTTTGAGACTCAA
TTTATCATGTCTTCAAGACCTCTCTTGCAAGAAAACAACTGGCCAACACAGAATCCCATACCAGAGGATG
GGATGTATTCTTCATACTTCACCCACAGAAGTTCTCCTTCAGAAATGGATGAAAACGAACTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201692 representing NM_145133
Red=Cloning site Green=Tags(s)

MSTFEDADTEETVTCLQMTIYHPGQQSGIFKSIRFCSKEKFPSIEVVKFGRNSNMCQYTFQDKQVSRIQF
VLQPFKQFNSSVLSFEIKNMSKKTSLMVDNQELGYLNKMDLPYKCMLRFGEYQFLLQKEDGESVESFETQ
FIMSSRPLLQENNWPTQNPIPEDGMYSSYFTHRSSPSEMDENEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145133
ORF Size 552 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145133.4
RefSeq Size 2151 bp
RefSeq ORF 555 bp
Locus ID 211550
UniProt ID Q793I8
Cytogenetics 3 G2
Summary Adapter molecule that plays a key role in the activation of proinflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs) (PubMed:11798190). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism (By similarity). TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of proinflammatory NF-kappa-B signaling (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145133" in other vectors (4)
SKU Description Size Price
MC207861 Tifa (untagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa), (10ug) 10 ug
$330.00
MR201692 Tifa (Myc-DDK-tagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa) 10 ug
$289.00 $300.00
MR201692L3 Lenti ORF clone of Tifa (Myc-DDK-tagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa) 10 ug
$600.00
MR201692L4 Lenti ORF clone of Tifa (mGFP-tagged) - Mouse TRAF-interacting protein with forkhead-associated domain (Tifa) 10 ug
$600.00

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