Chac2 (NM_026527) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201563
Chac2 (tGFP-tagged) - Mouse ChaC, cation transport regulator homolog 2 (E. coli) (Chac2)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Chac2
Synonyms 2510006C20Rik; RP23-20N14.6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201563 representing NM_026527
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGTGTTTGGTTATGGGTCCCTCATCTGGAAGGTGGACTTCCCCTACCAGGACAAGCTGGTTGGTT
ACATCACAAACTACAGTCGGCGCTTCTGGCAAGGTAGCACGGACCACCGCGGGGTCCCAGGCAAGCCTGG
AAGAGTTGTGACCCTTGTTGAAGATCCTGGGGGCAGTGTATGGGGTGTTGCTTACAAACTACCAGTAGGA
AAAGAAGAGGAAGTAAAAACATACCTTGACTTCAGAGAGAAAGGAGGCTACAGAACTACGACAGTCATTT
TTTATCCAAAAGATTCCACAACAAAACCATTCAGTGTTTTGCTCTACATTGGAACATATGATAATCCTAA
CTATCTTGGCCCTGCACCTCTGGAAGATATTGCTGAACAAATTTTTAATGCAGCTGGTCCAAGTGGAAGA
AATACAGAATATCTTTTTGAACTTGCAGATTCTGTTAGGAAACTTGTACCAGAAGATGCAGACGAGCATC
TCTTCTCTTTGGAAAAATTAGTAAAGGAACGCTTAGAAGGAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201563 representing NM_026527
Red=Cloning site Green=Tags(s)

MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVG
KEEEVKTYLDFREKGGYRTTTVIFYPKDSTTKPFSVLLYIGTYDNPNYLGPAPLEDIAEQIFNAAGPSGR
NTEYLFELADSVRKLVPEDADEHLFSLEKLVKERLEGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026527
ORF Size 534 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026527.2, NP_080803.1
RefSeq Size 1328 bp
RefSeq ORF 537 bp
Locus ID 68044
UniProt ID Q9CQG1
Cytogenetics 11 A4
Summary Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide (PubMed:27913623). Acts specifically on glutathione, but not on other gamma-glutamyl peptides (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026527" in other vectors (4)
SKU Description Size Price
MC202203 Chac2 (untagged) - Mouse ChaC, cation transport regulator homolog 2 (E. coli) (Chac2), (10ug) 10 ug
$300.00
MR201563 Chac2 (Myc-DDK-tagged) - Mouse ChaC, cation transport regulator homolog 2 (E. coli) (Chac2) 10 ug
$289.00 $300.00
MR201563L3 Lenti ORF clone of Chac2 (Myc-DDK-tagged) - Mouse ChaC, cation transport regulator homolog 2 (E. coli) (Chac2) 10 ug
$600.00
MR201563L4 Lenti ORF clone of Chac2 (mGFP-tagged) - Mouse ChaC, cation transport regulator homolog 2 (E. coli) (Chac2) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.