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Siva1 (NM_013929) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201528
Siva1 (tGFP-tagged) - Mouse SIVA1, apoptosis-inducing factor (Siva1)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Siva1
Synonyms CD27bp; Siva
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201528 representing NM_013929
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAGCGGAGCTGCCCGTTCGCAGACGCAGCCCCGCTCCAACTCAAAGTCCACGTGGGCCTGAAAG
AGCTGAGCCACGGTGTGTTCGCCGAGCGCTACTCACGCGAGGTCTTCGAAAGAACCAAGCAGCTCCTTTT
CCAAGGGGCTCGGGCCTATAGAGATCACATATCGAGCGAAGATTGTTCCGTGAACCACCTGCAGGAGTCA
CTGAAGTCTGGTGTGGTGGGAGCCCCTCAACCTGCGAGGGGACAGATGTTGATTGGACCTGATGGCCGAC
TGACACGGTGCCAAGCTCAGGCCTCAGAAGGTGGCCTTCCCAGGACAGCGCCCATCGCTTGTTCATCGTG
CATGAGATCTGTGGATGGGAAGGCGGCCTGCAGCCAGTGCGAGCGGGCCCTGTGTGGGCAGTGTGTATAC
ACCTGCTGGGGCTGCGGTGCTTTGGCCTGTGTGCTGTGTGGCCTTGCAGACTATGCCGATGATGGTGAGA
AGACACTGTGCACCAGCTGTGCTATGTTTGAAGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201528 representing NM_013929
Red=Cloning site Green=Tags(s)

MPKRSCPFADAAPLQLKVHVGLKELSHGVFAERYSREVFERTKQLLFQGARAYRDHISSEDCSVNHLQES
LKSGVVGAPQPARGQMLIGPDGRLTRCQAQASEGGLPRTAPIACSSCMRSVDGKAACSQCERALCGQCVY
TCWGCGALACVLCGLADYADDGEKTLCTSCAMFEA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013929
ORF Size 525 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013929.1, NP_038957.1
RefSeq Size 749 bp
RefSeq ORF 528 bp
Locus ID 30954
UniProt ID O54926
Cytogenetics 12 F1
Summary Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013929" in other vectors (4)
SKU Description Size Price
MC207482 Siva1 (untagged) - Mouse SIVA1, apoptosis-inducing factor (Siva1), transcript variant 1, (10ug) 10 ug
$330.00
MR201528 Siva1 (Myc-DDK-tagged) - Mouse SIVA1, apoptosis-inducing factor (Siva1), transcript variant 1 10 ug
$289.00 $300.00
MR201528L3 Lenti ORF clone of Siva1 (Myc-DDK-tagged) - Mouse SIVA1, apoptosis-inducing factor (Siva1), transcript variant 1 10 ug
$600.00
MR201528L4 Lenti ORF clone of Siva1 (mGFP-tagged) - Mouse SIVA1, apoptosis-inducing factor (Siva1), transcript variant 1 10 ug
$600.00

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