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H1f9 (NM_018792) Mouse Tagged ORF Clone

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SKU
MG201425
Hils1 (tGFP-tagged) - Mouse histone H1-like protein in spermatids 1 (Hils1)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol H1f9
Synonyms H1.9; Hi; Hils1; TIS; TISP64
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201425 representing NM_018792
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAAATGGTGGCTGGAGACCAAGATGCCGGCACACTGTGGGTCCCAAGCCAGAGTGAGAGTCAAA
CTGAGTCCGACATCAGTACCCAAAGCCTGCGGAAGCCCACCATGTCGTATGTGATTCTGAAGACTTTGGC
TGACAAGCGGGTACATAATTGTGTGTCCCTTGCTACCTTGAAGAAAGCTGTGTCTATCACAGGGTACAAT
ATGACCCATAACACCTGGCGCTTCAAGCGTGTGCTCCAGAATCTACTCGATAAAGGCATGATCATGCATG
TGACCTGCTGCAAGGGTGCCTCCGGCTCCCTCTGCCTGTGCAAGGAGCGGGCCCTCAAGTCCAACCACAG
GGCCAAGAGATGCCAGGACAGACAGAAGAGCCAGAAGCCTCAGAAGCCTGGGCAGCGTGAGTCTGAACCA
TGCCAATTGCTACTAAGCTCCAAAAAGAAGAATGACCAGCTTTTCAAAGGAGTCCGTAGGGTGGCCAAAG
GCAACCGCCATTGCCATTAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201425 representing NM_018792
Red=Cloning site Green=Tags(s)

MAQMVAGDQDAGTLWVPSQSESQTESDISTQSLRKPTMSYVILKTLADKRVHNCVSLATLKKAVSITGYN
MTHNTWRFKRVLQNLLDKGMIMHVTCCKGASGSLCLCKERALKSNHRAKRCQDRQKSQKPQKPGQRESEP
CQLLLSSKKKNDQLFKGVRRVAKGNRHCHY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018792
ORF Size 510 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018792.2, NP_061262.1
RefSeq Size 796 bp
RefSeq ORF 513 bp
Locus ID 54388
UniProt ID Q9QYL0
Cytogenetics 11 D
Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is expressed in nuclei of late maturing spermatids. provided by RefSeq, Nov 2015
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Documents
Resources
"NM_018792" in other vectors (4)
SKU Description Size Price
MC209974 Hils1 (untagged) - Mouse histone H1-like protein in spermatids 1 (Hils1), (10ug) 10 ug
$330.00
MR201425 Hils1 (Myc-DDK-tagged) - Mouse histone H1-like protein in spermatids 1 (Hils1) 10 ug
$289.00 $300.00
MR201425L3 Lenti ORF clone of Hils1 (Myc-DDK-tagged) - Mouse histone H1-like protein in spermatids 1 (Hils1) 10 ug
$600.00
MR201425L4 Lenti ORF clone of Hils1 (mGFP-tagged) - Mouse histone H1-like protein in spermatids 1 (Hils1) 10 ug
$600.00

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