Pop5 (NM_026398) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201402
Pop5 (tGFP-tagged) - Mouse processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) (Pop5)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pop5
Synonyms 1500019J17Rik; 2700077E03Rik; AW049989; Rnasep3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201402 representing NM_026398
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCGGTTCAAGCACAGGTACCTTCTGTGCGAGCTGGTGTCCGAGGACGCGCGCTGCCGTTTGAGCC
TGGACGACCGCGTGCTGGGCGGGCTGGTCCGCGACACGATCGCCCGGGTGCACGGGGCCTTCGGCGCCGC
CGCCTGCTCCGTGGGCTTTGCAGTCCGATACCTCAATGCCTACACTGGAGTCGTGCTACTGCGATGCCGG
AAGGATTTCTACCAGCTCGTGTGGTCAGCTCTTCCTTTCATCACCTATTTGGAGAACAAAGGCCACCGTT
ACCCGTGCTTCTTCAACACATTACACGTGGGAGGTACCATCAGAACCTGTCAGAAGTTCCTGATCCAGTA
CAACAGGAGACAGCTGCTGATCTTGTTGCAGAATTGCACTGACGAAGGAGAGCGGGAAGCCATCAAGAAG
TCTGTCTCAAGAAGCTGTCTGCTGGACAGAGAGCCCGTTGAAGAGCTTTCAGACAGTGCTGGTGAGGAGG
TTGCAGAAGCAATGGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201402 representing NM_026398
Red=Cloning site Green=Tags(s)

MVRFKHRYLLCELVSEDARCRLSLDDRVLGGLVRDTIARVHGAFGAAACSVGFAVRYLNAYTGVVLLRCR
KDFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRRQLLILLQNCTDEGEREAIKK
SVSRSCLLDREPVEELSDSAGEEVAEAME

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026398
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026398.4
RefSeq Size 1030 bp
RefSeq ORF 510 bp
Locus ID 117109
UniProt ID Q9DB28
Cytogenetics 5 F
Summary Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026398" in other vectors (4)
SKU Description Size Price
MC202992 Pop5 (untagged) - Mouse processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) (Pop5), (10ug) 10 ug
$300.00
MR201402 Pop5 (Myc-DDK-tagged) - Mouse processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) (Pop5) 10 ug
$289.00 $300.00
MR201402L3 Lenti ORF clone of Pop5 (Myc-DDK-tagged) - Mouse processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) (Pop5) 10 ug
$600.00
MR201402L4 Lenti ORF clone of Pop5 (mGFP-tagged) - Mouse processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) (Pop5) 10 ug
$600.00

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