Cfl2 (NM_007688) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201352
Cfl2 (tGFP-tagged) - Mouse cofilin 2, muscle (Cfl2)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cfl2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201352 representing NM_007688
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCTGGAGTTACAGTGAATGATGAAGTCATCAAAGTTTTTAATGATATGAAAGTAAGAAAATCTT
CTACACAGGAGGAGATCAAAAAAAGAAAGAAAGCAGTTCTCTTCTGTTTGAGCGATGACAAAAGACAAAT
AATTGTAGAGGAAGCCAAGCAGATCTTGGTGGGTGACATTGGTGACACTGTAGAGGACCCCTACACATCT
TTTGTGAAGTTGCTACCTCTGAATGATTGCCGATATGCTTTGTACGATGCCACATACGAAACAAAAGAGT
CTAAGAAAGAAGACCTAGTATTTATATTCTGGGCTCCTGAAAGTGCACCGTTAAAAAGCAAGATGATTTA
TGCTAGCTCTAAAGATGCCATTAAAAAGAAATTTACAGGTATTAAACATGAGTGGCAAGTAAATGGCTTG
GACGATATTAAGGACCGCTCGACGCTGGGAGAGAAACTGGGGGGCAGTGTTGTAGTTTCCCTTGAAGGAA
AGCCACTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201352 representing NM_007688
Red=Cloning site Green=Tags(s)

MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTS
FVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGL
DDIKDRSTLGEKLGGSVVVSLEGKPL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007688
ORF Size 498 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007688.2, NP_031714.1
RefSeq Size 2940 bp
RefSeq ORF 501 bp
Locus ID 12632
UniProt ID P45591
Cytogenetics 12 C1
Summary Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007688" in other vectors (4)
SKU Description Size Price
MC205639 Cfl2 (untagged) - Mouse cofilin 2, muscle (Cfl2), (10ug) 10 ug
$300.00
MR201352 Cfl2 (Myc-DDK-tagged) - Mouse cofilin 2, muscle (Cfl2) 10 ug
$289.00 $300.00
MR201352L3 Lenti ORF clone of Cfl2 (Myc-DDK-tagged) - Mouse cofilin 2, muscle (Cfl2) 10 ug
$600.00
MR201352L4 Lenti ORF clone of Cfl2 (mGFP-tagged) - Mouse cofilin 2, muscle (Cfl2) 10 ug
$600.00

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