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Ppia (NM_008907) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201314
Ppia (tGFP-tagged) - Mouse peptidylprolyl isomerase A (Ppia)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $489.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppia
Synonyms 2700098C05; Cphn; CyP-18; CypA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201314 representing NM_008907
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCAACCCCACCGTGTTCTTCGACATCACGGCCGATGACGAGCCCTTGGGCCGCGTCTCCTTCGAGC
TGTTTGCAGACAAAGTTCCAAAGACAGCAGAAAACTTTCGAGCTCTGAGCACTGGAGAGAAAGGATTTGG
CTATAAGGGTTCCTCCTTTCACAGAATTATTCCAGGATTCATGTGCCAGGGTGGTGACTTTACACGCCAT
AATGGCACTGGCGGCAGGTCCATCTACGGAGAGAAATTTGAGGATGAGAACTTCATCCTAAAGCATACAG
GTCCTGGCATCTTGTCCATGGCAAATGCTGGACCAAACACAAACGGTTCCCAGTTTTTTATCTGCACTGC
CAAGACTGAATGGCTGGATGGCAAGCATGTGGTCTTTGGGAAGGTGAAAGAAGGCATGAACATTGTGGAA
GCCATGGAGCGTTTTGGGTCCAGGAATGGCAAGACCAGCAAGAAGATCACCATTTCCGACTGTGGACAGC
TC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201314 representing NM_008907
Red=Cloning site Green=Tags(s)

MVNPTVFFDITADDEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSSFHRIIPGFMCQGGDFTRH
NGTGGRSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVE
AMERFGSRNGKTSKKITISDCGQL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008907
ORF Size 492 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008907.2
RefSeq Size 736 bp
RefSeq ORF 495 bp
Locus ID 268373
UniProt ID P17742
Cytogenetics 11 3.97 cM
Summary PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008907" in other vectors (4)
SKU Description Size Price
MC202774 Ppia (untagged) - Mouse peptidylprolyl isomerase A (Ppia), (10ug) 10 ug
$150.00
MR201314 Ppia (Myc-DDK-tagged) - Mouse peptidylprolyl isomerase A (Ppia) 10 ug
$289.00
MR201314L3 Lenti ORF clone of Ppia (Myc-DDK-tagged) - Mouse peptidylprolyl isomerase A (Ppia) 10 ug
$450.00
MR201314L4 Lenti ORF clone of Ppia (mGFP-tagged) - Mouse peptidylprolyl isomerase A (Ppia) 10 ug
$450.00

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