Gadd45b (NM_008655) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201229
Gadd45b (tGFP-tagged) - Mouse growth arrest and DNA-damage-inducible 45 beta (Gadd45b)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gadd45b
Synonyms AI323528; Myd118
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201229 representing NM_008655
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGGAAGAGCTGGTGGCGAGCGACAACGCGGTTCAGAAGATGCAGGCGGTGACTGCCGCGGTGG
AGCAGCTGCTGGTGGCCGCGCAGCGTCAGGATCGCCTCACCGTGGGGGTGTACGAGGCGGCCAAACTGAT
GAATGTGGACCCCGACAGCGTGGTCTTGTGCCTCCTGGCCATAGACGAAGAAGAGGAGGATGATATCGCT
CTGCAGATTCACTTCACCCTGATCCAGTCGTTCTGCTGCGACAATGACATTGACATCGTCCGGGTATCAG
GCATGCAGAGGCTGGCGCAGCTCCTGGGGGAGCCGGCGGAGACATTGGGCACAACCGAAGCCCGAGACCT
GCACTGCCTCCTGGTCACGAACTGTCATACAGATTCCTGGAAAAGCCAAGGCTTGGTGGAGGTGGCCAGT
TACTGTGAAGAGAGCAGAGGCAATAACCAATGGGTCCCCTATATCTCTCTAGAGGAACGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201229 representing NM_008655
Red=Cloning site Green=Tags(s)

MTLEELVASDNAVQKMQAVTAAVEQLLVAAQRQDRLTVGVYEAAKLMNVDPDSVVLCLLAIDEEEEDDIA
LQIHFTLIQSFCCDNDIDIVRVSGMQRLAQLLGEPAETLGTTEARDLHCLLVTNCHTDSWKSQGLVEVAS
YCEESRGNNQWVPYISLEER

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008655
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008655.1, NP_032681.1
RefSeq Size 1305 bp
RefSeq ORF 483 bp
Locus ID 17873
UniProt ID P22339
Cytogenetics 10 39.72 cM
Summary Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008655" in other vectors (4)
SKU Description Size Price
MC204491 Gadd45b (untagged) - Mouse growth arrest and DNA-damage-inducible 45 beta (Gadd45b), (10ug) 10 ug
$150.00
MR201229 Gadd45b (Myc-DDK-tagged) - Mouse growth arrest and DNA-damage-inducible 45 beta (Gadd45b) 10 ug
$289.00
MR201229L3 Lenti ORF clone of Gadd45b (Myc-DDK-tagged) - Mouse growth arrest and DNA-damage-inducible 45 beta (Gadd45b) 10 ug
$450.00
MR201229L4 Lenti ORF clone of Gadd45b (mGFP-tagged) - Mouse growth arrest and DNA-damage-inducible 45 beta (Gadd45b) 10 ug
$450.00

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