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Ndufaf5 (NM_027093) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201174
Ndufaf5 (tGFP-tagged) - Mouse RIKEN cDNA 2310003L22 gene (2310003L22Rik)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Ndufaf5 or NM_027093"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndufaf5
Synonyms 2310003L22Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201174 representing NM_027093
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGAGGCGACACACTCTATGAACTCCGGTGTTCATTACAGCTAGCAGAAACTGAAAGGGAAGGAG
GCTTTTCCCCACATATTTCTCCTTTCACTGCTGTCAATGACCTAGGGCATCTGCTGGGAAGAGCTGGCTT
CAACACTCTGACTGTGGATACTGATGAAATTCAAGTTAACTATCCTGGAATGTTTGAATTGATGGAAGAT
TTAAAAGGTATGGGTGAGAGCAACTGTTCTTGGAACAGAAAAGCCCTGCTGCACCGAGACACAATGCTGG
CAGCTGCAGCGGTTTACAGAGAAATGTACCGGAATGAGGATGGTTCCATACCTGCCACCTTTCAGATCTA
CCACATGATAGGATGGAAATACCATGACTCTCAGGCAAGGCCAGCTGAAAGAGGTTCTGCAACCGTGTCA
TTTGGAGAGCTAGCAAAACTAAATGATGTCATGTCGCATGAGAAAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201174 representing NM_027093
Red=Cloning site Green=Tags(s)

MFGGDTLYELRCSLQLAETEREGGFSPHISPFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELMED
LKGMGESNCSWNRKALLHRDTMLAAAAVYREMYRNEDGSIPATFQIYHMIGWKYHDSQARPAERGSATVS
FGELAKLNDVMSHEKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027093
ORF Size 468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027093.3
RefSeq Size 1179 bp
RefSeq ORF 1032 bp
Locus ID 69487
UniProt ID A2APY7
Cytogenetics 2 F3
Summary Arginine hydroxylase involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. Acts by mediating hydroxylation of 'Arg-111' of NDUFS7. May also have methyltransferase activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027093" in other vectors (4)
SKU Description Size Price
MC205624 Ndufaf5 (untagged) - Mouse RIKEN cDNA 2310003L22 gene (2310003L22Rik), (10ug) 10 ug
$150.00
MR201174 Ndufaf5 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310003L22 gene (2310003L22Rik) 10 ug
$289.00
MR201174L3 Lenti ORF clone of Ndufaf5 (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310003L22 gene (2310003L22Rik) 10 ug
$450.00
MR201174L4 Lenti ORF clone of Ndufaf5 (mGFP-tagged) - Mouse RIKEN cDNA 2310003L22 gene (2310003L22Rik) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.