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Snrnp27 (NM_025665) Mouse Tagged ORF Clone

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SKU
MG201154
Snrnp27 (tGFP-tagged) - Mouse RIKEN cDNA 2610209M04 gene (2610209M04Rik)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Snrnp27
Synonyms 2610209M04Rik; AI449063; AU022368; Ry1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201154 representing NM_025665
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCGAAGTCGTAGCCGCTCCCCGCGTAGGGAACGTAGGCGGTCCCGGTCCACATCTCGGGATCGAG
AAAGAAGACGCCGAGAAAGGTCCAGGTCCAGGGAGAGAGATCGCAGAAGGAGCCGCTCAAGATCTCCACA
CCGAAGACGCTCCAGGTCTCCAAGACGACATAGATCCACATCTCCCTCCCCTTCTCGACTGAAAGAAAGA
AGAGATGAGGAAAAGAAAGAAACTAAAGAAATAAAGAACAAAGAACGGCAGATCACTGAGGAAGACCTGG
AGGGTAAAACAGAGGAAGAAATAGAAATGATGAAATTAATGGGATTTGCCTCTTTTGACTCCACAAAAGG
GAAGAAGGTAGACGGCTCTGTAAATGCCTATGCCATCAACGTGTCTCAAAAGAGGAAGTACAGGCAGTAC
ATGAATCGAAAAGGCGGATTCAACAGACCTCTGGATTTTATTGCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201154 representing NM_025665
Red=Cloning site Green=Tags(s)

MGRSRSRSPRRERRRSRSTSRDRERRRRERSRSRERDRRRSRSRSPHRRRSRSPRRHRSTSPSPSRLKER
RDEEKKETKEIKNKERQITEEDLEGKTEEEIEMMKLMGFASFDSTKGKKVDGSVNAYAINVSQKRKYRQY
MNRKGGFNRPLDFIA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025665
ORF Size 465 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025665.2
RefSeq Size 837 bp
RefSeq ORF 468 bp
Locus ID 66618
UniProt ID Q8K194
Cytogenetics 6 D1
Summary May play a role in mRNA splicing.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025665" in other vectors (4)
SKU Description Size Price
MC201415 Snrnp27 (untagged) - Mouse small nuclear ribonucleoprotein 27 (U4/U6.U5) (Snrnp27), (10ug) 10 ug
$150.00
MR201154 Snrnp27 (Myc-DDK-tagged) - Mouse small nuclear ribonucleoprotein 27 (U4/U6.U5) (Snrnp27) 10 ug
$289.00
MR201154L3 Lenti ORF clone of Snrnp27 (Myc-DDK-tagged) - Mouse small nuclear ribonucleoprotein 27 (U4/U6.U5) (Snrnp27) 10 ug
$450.00
MR201154L4 Lenti ORF clone of Snrnp27 (mGFP-tagged) - Mouse small nuclear ribonucleoprotein 27 (U4/U6.U5) (Snrnp27) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.