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Fgf1 (NM_010197) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201152
Fgf1 (tGFP-tagged) - Mouse fibroblast growth factor 1 (Fgf1)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $489.00
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Other products for "Fgf1 or NM_010197"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fgf1
Synonyms Dffrx; Fam; Fgf-1; Fgfa
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010197
ORF Size 465 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010197.3, NP_034327.1
RefSeq Size 3909 bp
RefSeq ORF 468 bp
Locus ID 14164
UniProt ID P61148
Cytogenetics 18 20.74 cM
Summary Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.UniProtKB/Swiss-Prot Function
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Resources
"NM_010197" in other vectors (4)
SKU Description Size Price
MC203263 Fgf1 (untagged) - Mouse fibroblast growth factor 1 (Fgf1), (10ug) 10 ug
$150.00
MR201152 Fgf1 (Myc-DDK-tagged) - Mouse fibroblast growth factor 1 (Fgf1) 10 ug
$289.00
MR201152L3 Lenti ORF clone of Fgf1 (Myc-DDK-tagged) - Mouse fibroblast growth factor 1 (Fgf1) 10 ug
$450.00
MR201152L4 Lenti ORF clone of Fgf1 (mGFP-tagged) - Mouse fibroblast growth factor 1 (Fgf1) 10 ug
$450.00

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