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Arpc5l (NM_028809) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201112
Arpc5l (tGFP-tagged) - Mouse actin related protein 2/3 complex, subunit 5-like (Arpc5l)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arpc5l
Synonyms 2010015J01Rik; AI852867; ARC16-2; AW555592; AW742746
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201112 representing NM_028809
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCGGAACACACTGTCCTCACGCTTCCGCCGCGTGGATATCGACGAATTTGACGAGAACAAATTCG
TAGACGAGCACGAAGAGGCAGCGGCGGCGGCGGGCGAGCCAGGCCCCGACCCCTGCGAGGTAGACGGGCT
CCTGCGGCAAGGGGACATGCTTCGGGCGTTCCATGCAGCTTTGCGGAACTCACCAATCAACACCAAGAAT
CAAGCTGTGAAGGAACGAGCCCAGGGTGTAGTCCTGAAAGTACTCACAAACTTTAAGAGCAGCGAAATTG
AGCAGGCTGTGCAGTCACTGGACAGAAATGGCATTGATTTGCTAATGAAGTACATTTATAAAGGGTTTGA
GAAGCCTACAGAAAATAGCAGTGCAGTGCTACTCCAGTGGCATGAAAAGGCCTTAGCTGTAGGAGGACTA
GGCTCCATTATAAGAGTTCTTACAGCAAGAAAGACTGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201112 representing NM_028809
Red=Cloning site Green=Tags(s)

MARNTLSSRFRRVDIDEFDENKFVDEHEEAAAAAGEPGPDPCEVDGLLRQGDMLRAFHAALRNSPINTKN
QAVKERAQGVVLKVLTNFKSSEIEQAVQSLDRNGIDLLMKYIYKGFEKPTENSSAVLLQWHEKALAVGGL
GSIIRVLTARKTV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028809
ORF Size 459 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028809.1, NP_083085.1
RefSeq Size 1271 bp
RefSeq ORF 462 bp
Locus ID 74192
UniProt ID Q9D898
Cytogenetics 2 B
Summary May function as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028809" in other vectors (4)
SKU Description Size Price
MC207741 Arpc5l (untagged) - Mouse actin related protein 2/3 complex, subunit 5-like (Arpc5l), (10ug) 10 ug
$165.00
MR201112 Arpc5l (Myc-DDK-tagged) - Mouse actin related protein 2/3 complex, subunit 5-like (Arpc5l) 10 ug
$289.00
MR201112L3 Lenti ORF clone of Arpc5l (Myc-DDK-tagged) - Mouse actin related protein 2/3 complex, subunit 5-like (Arpc5l) 10 ug
$450.00
MR201112L4 Lenti ORF clone of Arpc5l (mGFP-tagged) - Mouse actin related protein 2/3 complex, subunit 5-like (Arpc5l) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.