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Ccdc184 (NM_177716) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201100
AI836003 (tGFP-tagged) - Mouse expressed sequence AI836003 (AI836003)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ccdc184
Synonyms 5630400A08; AI836003
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201100 representing NM_177716
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAACTGATGGAGCACCTGAAGGCCCAGCTGCAGGCCCTGTTTGAGGACGTGAGGGCCATGCGAG
GGGCACTGGACGAGCAGGCCTCGCACATCCAGGTTCTGTCGGACGACGTGTGCGCCAACCAGCGGGCTAT
AGTCTCCATGTGCCAGATTATGACCACTGCGCCCCGCCAGGGAGGCTTGGGCGTGGCCGGCGGCAAGGGG
AGCTTCCCAAGTGTTCCCCATGAACCAGAGACACCTTCGCCTGGGATCGAGGACAGCGGCTTGCTGGGTC
GTGATCCTGATGAAGACGAGGACGAAGAGGAGAAAGAGACACCTAACCCCGCCACACCCACCAGTCACTG
CGAGCTCTCTGAGAGCCCCGGTCTCCTTGGGGAGGATGGGCCACTTGTGGAGCCCCCCGACCTTCCTGAC
ATTACCCTGCTGCAGCTGGAGGGCGAGGCCTCTCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201100 representing NM_177716
Red=Cloning site Green=Tags(s)

MKELMEHLKAQLQALFEDVRAMRGALDEQASHIQVLSDDVCANQRAIVSMCQIMTTAPRQGGLGVAGGKG
SFPSVPHEPETPSPGIEDSGLLGRDPDEDEDEEEKETPNPATPTSHCELSESPGLLGEDGPLVEPPDLPD
ITLLQLEGEASL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177716
ORF Size 456 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177716.1, NP_808384.1
RefSeq Size 2471 bp
RefSeq ORF 576 bp
Locus ID 239650
UniProt ID Q8BMK5
Cytogenetics 15 F1
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"NM_177716" in other vectors (4)
SKU Description Size Price
MC207925 AI836003 (untagged) - Mouse expressed sequence AI836003 (AI836003), (10ug) 10 ug
$330.00
MR201100 AI836003 (Myc-DDK-tagged) - Mouse expressed sequence AI836003 (AI836003) 10 ug
$289.00
MR201100L3 Lenti ORF clone of AI836003 (Myc-DDK-tagged) - Mouse expressed sequence AI836003 (AI836003) 10 ug
$450.00
MR201100L4 Lenti ORF clone of AI836003 (mGFP-tagged) - Mouse expressed sequence AI836003 (AI836003) 10 ug
$450.00

Plasmid Map for MG201100

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG201100
Circular map for MG201100

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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