Sys1 (NM_025575) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201074
Sys1 (tGFP-tagged) - Mouse RIKEN cDNA 2610042O14 gene (2610042O14Rik)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sys1
Synonyms 2610042O14Rik; AI266964; C87389
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201074 representing NM_025575
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCCAGTTCCGCAGCTACGTGTGGGACCCGTTGCTAATCCTGTCGCAGATCGTACTCATGCAGA
CCGTCTACTATGGCTCTCTGGGCCTGTGGCTGGCGCTGGTGGACGCGCTGGTGCGCAGCAGCCCGTCCCT
GGACCAGATGTTCGACGCGGAGATCCTGGGCTTCTCCACCCCTCCAGGCCGGCTCTCAATGATGTCCTTC
GTCCTCAACGCCCTCACCTGTGCCCTGGGCTTGCTGTACTTCATCCGGCGAGGGAAGCAGTGCCTGGATT
TCACTGTCACTGTGCATTTCTTTCACCTCCTGGGCTGCTGGCTCTACAGCTCCCGTTTCCCCTCGGCGCT
GACCTGGTGGCTGGTCCAGGCTGTGTGCATTGCACTCATGGCCGTCATCGGGGAGTACCTGTGCATGCGG
ACGGAGCTCAAGGAGATCCCCCTCAGCTCAGCCCCTAAGTCCAATGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201074 representing NM_025575
Red=Cloning site Green=Tags(s)

MAGQFRSYVWDPLLILSQIVLMQTVYYGSLGLWLALVDALVRSSPSLDQMFDAEILGFSTPPGRLSMMSF
VLNALTCALGLLYFIRRGKQCLDFTVTVHFFHLLGCWLYSSRFPSALTWWLVQAVCIALMAVIGEYLCMR
TELKEIPLSSAPKSNV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025575
ORF Size 468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025575.3, NP_079851.2
RefSeq Size 1623 bp
RefSeq ORF 471 bp
Locus ID 66460
UniProt ID Q78S06
Cytogenetics 2 H3
Summary Involved in protein trafficking. May serve as a receptor for ARFRP1 (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025575" in other vectors (4)
SKU Description Size Price
MC204972 Sys1 (untagged) - Mouse SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (Sys1), (10ug) 10 ug
$150.00
MR201074 Sys1 (Myc-DDK-tagged) - Mouse SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (Sys1) 10 ug
$289.00
MR201074L3 Lenti ORF clone of Sys1 (Myc-DDK-tagged) - Mouse SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (Sys1) 10 ug
$450.00
MR201074L4 Lenti ORF clone of Sys1 (mGFP-tagged) - Mouse SYS1 Golgi-localized integral membrane protein homolog (S. cerevisiae) (Sys1) 10 ug
$450.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.