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Myl6 (BC081470) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201072
Myl6 (tGFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (cDNA clone MGC:102200 IMAGE:6824167), complete
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Myl6
Synonyms ESMLC, LC17A, LC17B, MLC1SM, MLC3SM, LC17-GI, LC17-NM, MLC3NM
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201072 representing BC081470
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGACTTCACCGAGGACCAGACCGCAGAATTCAAGGAGGCTTTCCAGCTGTTTGACCGAACAGGTG
ATGGCAAGATCCTGTACAGCCAGTGTGGGGATGTGATGCGGGCCCTGGGCCAGAACCCTACCAACGCCGA
GGTGCTCAAGGTCCTAGGGAACCCCAAGAGTGATGAGATGAATGTGAAGGTGCTGGACTTTGAGCACTTC
CTGCCCATGCTGCAGACCGTGGCCAAGAACAAGGACCAGGGAACCTACGAGGATTATGTTGAAGGCCTTC
GTGTGTTTGACAAGGAAGGAAATGGCACCGTCATGGGTGCTGAAATCCGTCATGTCCTAGTCACACTGGG
CGAGAAGATGACAGAGGAAGAAGTAGAGATGCTAGTGGCGGGGCATGAGGACAGCAATGGTTGCATCAAC
TATGAAGAGCTTGTCCGGATGGTGCTGAATGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201072 representing BC081470
Red=Cloning site Green=Tags(s)

MCDFTEDQTAEFKEAFQLFDRTGDGKILYSQCGDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHF
LPMLQTVAKNKDQGTYEDYVEGLRVFDKEGNGTVMGAEIRHVLVTLGEKMTEEEVEMLVAGHEDSNGCIN
YEELVRMVLNG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC081470
ORF Size 453 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC081470, AAH81470
RefSeq Size 646 bp
RefSeq ORF 455 bp
Locus ID 17904
Cytogenetics 10 D3
Summary Regulatory light chain of myosin. Does not bind calcium.UniProtKB/Swiss-Prot Function
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"BC081470" in other vectors (3)
SKU Description Size Price
MR201072 Myl6 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (cDNA clone MGC:102200 IMAGE:6824167), complete 10 ug
$289.00
MR201072L3 Lenti ORF clone of Myl6 (Myc-DDK-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (cDNA clone MGC:102200 IMAGE:6824167), complete 10 ug
$450.00
MR201072L4 Lenti ORF clone of Myl6 (mGFP-tagged) - Mouse myosin, light polypeptide 6, alkali, smooth muscle and non-muscle (cDNA clone MGC:102200 IMAGE:6824167), complete 10 ug
$450.00

Plasmid Map for MG201072

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file-download TXT (FASTA format)

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Cloning scheme for MG201072
Circular map for MG201072

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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