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Cdc42ep5 (NM_021454) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201066
Cdc42ep5 (tGFP-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdc42ep5
Synonyms 1700027J19Rik; 2010007O02Rik; Borg3; C85526; CEP5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201066 representing NM_021454
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGTAATGAAGCAGCTGGGCCCTGCACAACCCAAGAAGCGGCTGGATCGCGGAGCACTCTCGATCT
CAGCACCCCTCGGAGACTTCAGGCACACATTGCACGTGGGACGCGGTGGCGACGCCTTCGGGGACACCTC
ATTCCTGAGTCGCCACGGGGGCGGTCCGCCTCCCGAGCCTGGAGCCCCACCGGTTGTGGCCCCACATTCT
GTCGCTCCACCTGCAGCCCCACAGCCCCCTGTGGCTGTGCCTTCACCTGCGGACCCGCTGCTGTCTTTCC
ACCTGGACCTGGGCCCCTCTATGCTGGACGCGGTGTTAGGCGTCATGGACGCGGAGCGCAGCGAGACAAC
AGCCACCAAGCCCGATGGGGATGCCCACCCTAGAGTGCAGCACCCCAAGACCCGCTGCTGCTCAAATGCT
GACCTCCAGCTGGACGACGTCATCGGTCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201066 representing NM_021454
Red=Cloning site Green=Tags(s)

MPVMKQLGPAQPKKRLDRGALSISAPLGDFRHTLHVGRGGDAFGDTSFLSRHGGGPPPEPGAPPVVAPHS
VAPPAAPQPPVAVPSPADPLLSFHLDLGPSMLDAVLGVMDAERSETTATKPDGDAHPRVQHPKTRCCSNA
DLQLDDVIGL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021454
ORF Size 450 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021454.4
RefSeq Size 867 bp
RefSeq ORF 453 bp
Locus ID 58804
UniProt ID Q9Z0X0
Cytogenetics 7 A1
Summary Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts. Inhibits MAPK8 independently of CDC42 binding. Controls septin organization and this effect is negatively regulated by CDC42.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021454" in other vectors (3)
SKU Description Size Price
MR201066 Cdc42ep5 (Myc-DDK-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5) 10 ug
$289.00
MR201066L3 Lenti ORF clone of Cdc42ep5 (Myc-DDK-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5) 10 ug
$450.00
MR201066L4 Lenti ORF clone of Cdc42ep5 (mGFP-tagged) - Mouse CDC42 effector protein (Rho GTPase binding) 5 (Cdc42ep5) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.