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Rpp21 (NM_026308) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201065
Rpp21 (tGFP-tagged) - Mouse ribonuclease P 21 subunit (human) (Rpp21)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpp21
Synonyms 0610037N01Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201065 representing NM_026308
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCCCAGTGAAGGACCGGGAAGCCTTCCAGAGACTCAGCTTCCTGTACCAGGCTGCACACTGCG
TCCTCTCTCAGAACCCCGAGAACCAGGCGCTGGCTAGGTTTTACTGCCACACAGAGAAAACCATCGCGAA
GCGGCTGGTCCTCCGGCAGGACCCCTCTGTGAAGAGGACGCTCTGTCGCAGCTGCTCCTCTCTCCTCATT
CCGGGCCTCACCTGCACGCAGCGCCAGAGACGCCGAAAGGGACAGCGCTGGACAGTACAGACCTGTCTGA
CATGCCAGCGGAGCCAGAGATTCCTCAATGACCCTAAGCATCTGCTCTGGGGTGACCGGCCTGAAGCCCA
GCTGGAAAATCAGGCAGACATCAATCCATCAGAGCCCCTGCCAAACATAGCTGACCTCCCCAAAGAGAAC
ATGCAGACTCAGGCTTTGAACACCAGTGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201065 representing NM_026308
Red=Cloning site Green=Tags(s)

MAGPVKDREAFQRLSFLYQAAHCVLSQNPENQALARFYCHTEKTIAKRLVLRQDPSVKRTLCRSCSSLLI
PGLTCTQRQRRRKGQRWTVQTCLTCQRSQRFLNDPKHLLWGDRPEAQLENQADINPSEPLPNIADLPKEN
MQTQALNTSE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026308
ORF Size 450 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026308.1, NP_080584.1
RefSeq Size 501 bp
RefSeq ORF 453 bp
Locus ID 67676
UniProt ID Q8R040
Cytogenetics 17 B1
Summary Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends.UniProtKB/Swiss-Prot Function
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"NM_026308" in other vectors (4)
SKU Description Size Price
MC204676 Rpp21 (untagged) - Mouse ribonuclease P 21 subunit (human) (Rpp21), (10ug) 10 ug
$150.00
MR201065 Rpp21 (Myc-DDK-tagged) - Mouse ribonuclease P 21 subunit (human) (Rpp21) 10 ug
$289.00
MR201065L3 Lenti ORF clone of Rpp21 (Myc-DDK-tagged) - Mouse ribonuclease P 21 subunit (human) (Rpp21) 10 ug
$450.00
MR201065L4 Lenti ORF clone of Rpp21 (mGFP-tagged) - Mouse ribonuclease P 21 subunit (human) (Rpp21) 10 ug
$450.00

Plasmid Map for MG201065

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG201065
Circular map for MG201065

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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