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Adm2 (NM_182928) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201053
Adm2 (tGFP-tagged) - Mouse adrenomedullin 2 (Adm2)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $489.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adm2
Synonyms Am2; IM; IMD; Imdn; inter
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201053 representing NM_182928
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGTTGCTGATGGTCACGGTAACCCTCGGTTGCATCAGCCTCCTCTACCTGCTCCCCGGCACGT
TGTCTGGCAGCCTGGGCAAGGGACTGAGGCACTCCAGACCCAGAGAGCCCCCAGCTAAGATTCCTTCCAG
TAACCTGCAGCCTGGACACCCTTCCCTTCAGCCTGTAGTCTGGAAGTCTCGTCGTCATGCCCCCCAGCCA
CAGGGAAGGGGCAACAGGGCCCTTGCTATGGTTCATCTGCCTCAGGGTGGTGGCTCACGACACCCTGGTC
CCCAGCGTCCCACGGGATCCCGAAGACCCCATGCCCAGCTCCTGCGGGTTGGCTGTGTACTGGGTACATG
CCAAGTCCAGAATCTTAGCCATCGCCTGTGGCAGCTTGTCCGGCCAGCTGGCCGGCGGGACTCAGCTCCT
GTGGATCCCAGCAGCCCCCACAGTTATGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201053 representing NM_182928
Red=Cloning site Green=Tags(s)

MAQLLMVTVTLGCISLLYLLPGTLSGSLGKGLRHSRPREPPAKIPSSNLQPGHPSLQPVVWKSRRHAPQP
QGRGNRALAMVHLPQGGGSRHPGPQRPTGSRRPHAQLLRVGCVLGTCQVQNLSHRLWQLVRPAGRRDSAP
VDPSSPHSYG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182928
ORF Size 450 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182928.5, NP_891558.1
RefSeq Size 1164 bp
RefSeq ORF 453 bp
Locus ID 223780
UniProt ID Q7TNK8
Cytogenetics 15 E3
Summary This gene encodes a member of the calcitonin gene-related peptide (CGRP)/calcitonin family of hormones that play a role in the regulation of cardiovascular homeostasis, prolactin release, anti-diuresis, anti-natriuresis, and regulation of food and water intake. The encoded protein is proteolytically processed to generate one or more biologically active peptides. Intravenous injection of the active peptide was found to protect mouse lungs from ischemia/reperfusion injury. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_182928" in other vectors (4)
SKU Description Size Price
MC212713 Adm2 (untagged) - Mouse adrenomedullin 2 (Adm2), (10ug) 10 ug
$165.00
MR201053 Adm2 (Myc-DDK-tagged) - Mouse adrenomedullin 2 (Adm2) 10 ug
$289.00
MR201053L3 Lenti ORF clone of Adm2 (Myc-DDK-tagged) - Mouse adrenomedullin 2 (Adm2) 10 ug
$450.00
MR201053L4 Lenti ORF clone of Adm2 (mGFP-tagged) - Mouse adrenomedullin 2 (Adm2) 10 ug
$450.00

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