Calm1 (NM_009790) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG201051
Calm1 (tGFP-tagged) - Mouse calmodulin 1 (Calm1)
  $489.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Calm1
Synonyms AI256814; AI327027; AI461935; AL024000; Calm; CaM; Cam1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201051 representing NM_009790
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGATCAGCTGACTGAAGAGCAGATTGCTGAATTCAAGGAAGCTTTCTCCCTATTCGATAAAGATG
GTGACGGCACCATCACAACCAAGGAACTGGGGACCGTCATGCGGTCACTGGGTCAGAACCCAACAGAAGC
CGAGCTGCAGGATATGATCAACGAAGTGGATGCTGATGGCAATGGCACCATTGACTTCCCAGAGTTCTTG
ACTATGATGGCTAGAAAAATGAAAGACACAGATAGCGAAGAAGAGATCCGCGAGGCCTTCCGAGTGTTTG
ACAAGGATGGGAATGGTTACATCAGTGCGGCAGAACTGCGCCACGTCATGACAAACTTAGGAGAAAAGCT
AACAGATGAAGAAGTAGATGAAATGATCAGAGAAGCAGATATTGATGGCGACGGACAAGTCAACTATGAA
GAATTCGTACAGATGATGACTGCAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201051 representing NM_009790
Red=Cloning site Green=Tags(s)

MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFL
TMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYE
EFVQMMTAK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009790
ORF Size 447 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009790.5
RefSeq Size 4001 bp
RefSeq ORF 450 bp
Locus ID 12313
UniProt ID P0DP26
Cytogenetics 12 E
Summary This gene encodes a member of the EF-hand calcium-binding protein family. The encoded protein acts as a calcium sensor and is involved in relaying signals to calcium-sensitive proteins, enzymes and ion channels. The protein-calcium complex binds target proteins to regulate several cellular processes, including smooth muscle contraction, inflammation, apoptosis and the immune response. Mutations in the human gene are associated with catecholaminergic polymorphic ventricular tachycardia and long QT syndrome 14. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Sep 2015
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Documents
Resources
"NM_009790" in other vectors (6)
SKU Description Size Price
MC203458 Calm1 (untagged) - Mouse calmodulin 1 (Calm1), (10ug) 10 ug
$150.00
MR201051 Calm1 (Myc-DDK-tagged) - Mouse calmodulin 1 (Calm1) 10 ug
$289.00
MR201051L1 Lenti ORF clone of Calm1 (Myc-DDK-tagged) - Mouse calmodulin 1 (Calm1) 10 ug
$450.00
MR201051L2 Lenti ORF clone of Calm1 (mGFP-tagged) - Mouse calmodulin 1 (Calm1) 10 ug
$450.00
MR201051L3 Lenti ORF clone of Calm1 (Myc-DDK-tagged) - Mouse calmodulin 1 (Calm1) 10 ug
$450.00
MR201051L4 Lenti ORF clone of Calm1 (mGFP-tagged) - Mouse calmodulin 1 (Calm1) 10 ug
$450.00

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