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Edf1 (NM_021519) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG201022
Edf1 (tGFP-tagged) - Mouse endothelial differentiation-related factor 1 (Edf1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Edf1 or NM_021519"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Edf1
Synonyms 0610008L11Rik; AA409425
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG201022 representing NM_021519
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGAGTGACTGGGACACCGTGACGGTGCTGCGCAAGAAGGGCCCCACGGCCGCACAGGCCAAGT
CTAAGCAGGCCATCTTAGCGGCTCAGCGACGAGGAGAAGATGTGGAGACATCCAAGAAATGGGCTGCTGG
CCAGAACAAGCAGCATTCCATTACTAAAAACACAGCCAAGCTCGACCGGGAGACCGAGGAGCTGCATCAC
GACAGGGTGACCCTGGAGGTGGGCAAAGTGATCCAGCGGGGCCGGCAGAGCAAAGGCCTGACCCAGAAGG
ACCTGGCCACGAAAATCAATGAGAAGCCGCAAGTCATCGCAGACTATGAAAGTGGACGGGCCATTCCGAA
TAACCAGGTTTTGGGCAAAATTGAGAGAGCCATCGGCCTCAAGCTCCGGGGGAAGGACATCGGGAAGCCT
ATCGAGAAGGGGCCGAAGGCGAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG201022 representing NM_021519
Red=Cloning site Green=Tags(s)

MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQRRGEDVETSKKWAAGQNKQHSITKNTAKLDRETEELHH
DRVTLEVGKVIQRGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKP
IEKGPKAK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021519
ORF Size 444 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021519.2
RefSeq Size 678 bp
RefSeq ORF 447 bp
Locus ID 59022
UniProt ID Q9JMG1
Cytogenetics 2 A3
Summary Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. Might function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021519" in other vectors (3)
SKU Description Size Price
MR201022 Edf1 (Myc-DDK-tagged) - Mouse endothelial differentiation-related factor 1 (Edf1) 10 ug
$289.00
MR201022L3 Lenti ORF clone of Edf1 (Myc-DDK-tagged) - Mouse endothelial differentiation-related factor 1 (Edf1) 10 ug
$450.00
MR201022L4 Lenti ORF clone of Edf1 (mGFP-tagged) - Mouse endothelial differentiation-related factor 1 (Edf1) 10 ug
$450.00

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