Mrpl14 (NM_026732) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200958
Mrpl14 (tGFP-tagged) - Mouse mitochondrial ribosomal protein L14 (Mrpl14)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrpl14
Synonyms 1110006I11Rik; AI846816; MRP-L32; Rpml32
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200958 representing NM_026732
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCCTTACTGGGCTTTGGGGCTCTTTTGCCCATGTCAGCAGAGCATTCAGCCAGCGCTGCTTCA
GCACCTCTGGGAGCCTCAGTGCCGTCCAGAAGATGACTCGGGTCCGAGTAGTGGACAACAGCGCCCTGGG
CAGCACCCCATACCATCGGCCACCCCGGTGCATCCACGTCTATAACAAGAGTGGGGTGGGCAAGGTGGGC
GACCAGATCCTGCTGGCCATCAGGGGGCAGAAGAAGAAAGCACTCATCGTGGGACACCGCATGCCCGGCT
CCCGAATGACCCCAAAGTTTGACTCCAACAACGTGGTCCTCATTGAGGACAATGGCAACCCTGTGGGGAC
TCGAATTAAAATACCTATCCCGACCAGCCTTCGCAGAAGGGAAGGAGAGTATTCCAAGGTCCTGGCCATC
GCTCAGAACTTTGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200958 representing NM_026732
Red=Cloning site Green=Tags(s)

MAALTGLWGSFAHVSRAFSQRCFSTSGSLSAVQKMTRVRVVDNSALGSTPYHRPPRCIHVYNKSGVGKVG
DQILLAIRGQKKKALIVGHRMPGSRMTPKFDSNNVVLIEDNGNPVGTRIKIPIPTSLRRREGEYSKVLAI
AQNFV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026732
ORF Size 435 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026732.3
RefSeq Size 672 bp
RefSeq ORF 438 bp
Locus ID 68463
UniProt ID Q9D1I6
Cytogenetics 17 C
Summary May form part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1, intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026732" in other vectors (4)
SKU Description Size Price
MC204132 Mrpl14 (untagged) - Mouse mitochondrial ribosomal protein L14 (Mrpl14), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$150.00
MR200958 Mrpl14 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L14 (Mrpl14), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR200958L3 Lenti ORF clone of Mrpl14 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L14 (Mrpl14), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200958L4 Lenti ORF clone of Mrpl14 (mGFP-tagged) - Mouse mitochondrial ribosomal protein L14 (Mrpl14), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.