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Tomm22 (BC056920) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200911
Tomm22 (tGFP-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tomm22
Synonyms Tom22, 2310047D01
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200911 representing BC056920
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCGCCGTCGCTGCAGCCGGCGCTGGGGAGCCTCTGTCCCCCGAGGAATTACTCCCGAAAGCCG
AGGCAGAGAAAGCCGAGGAGGAGCTGGAAGAAGACGACGACGACGAGCTAGACGAGACCCTGTCGGAGAG
ACTCTGGGGTCTGACGGAGATGTTTCCCGAGAGGGTCCGGTCAGCCGCCGGAGCCACCTTTGACCTCTCG
CTCTTCGTGGCTCAGAAGATGTACAGATTTTCCAGGGCAGCTTTGTGGATTGGGACCACTTCCTTCATGA
TCCTGGTTCTTCCCGTTGTCTTTGAGACAGAAAAGTTGCAGATGGAGCAACAGCAGCAACTGCAGCAACG
GCAGATACTTTTAGGGCCTAACACAGGGCTGTCAGGAGGAATGCCAGGGGCTCTACCTCCACTTCCTGGA
AAGATG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200911 representing BC056920
Red=Cloning site Green=Tags(s)

MAAAVAAAGAGEPLSPEELLPKAEAEKAEEELEEDDDDELDETLSERLWGLTEMFPERVRSAAGATFDLS
LFVAQKMYRFSRAALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPNTGLSGGMPGALPPLPG
KM

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC056920
ORF Size 426 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC056920, AAH56920
RefSeq Size 1029 bp
RefSeq ORF 428 bp
Locus ID 223696
Cytogenetics 15 E1
Summary Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC056920" in other vectors (3)
SKU Description Size Price
MR200911 Tomm22 (Myc-DDK-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748) 10 ug
$289.00
MR200911L3 Lenti ORF clone of Tomm22 (Myc-DDK-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748) 10 ug
$450.00
MR200911L4 Lenti ORF clone of Tomm22 (mGFP-tagged) - Mouse translocase of outer mitochondrial membrane 22 homolog (yeast) (cDNA clone MGC:66499 IMAGE:6819748) 10 ug
$450.00

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