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Pomp (NM_025624) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200906
Pomp (tGFP-tagged) - Mouse proteasome maturation protein (Pomp)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pomp or NM_025624"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pomp
Synonyms 2510048O06Rik; Ump1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200906 representing NM_025624
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGCCAGAGGTCTTGGGTCGGAGCTGAAGGACAGTATTCCAGTCGCGGAGCTCTCAGCCAGCGGGC
CTTTCGAGAGTCACGATCTTCTCCGGAAAGGGTTTTCTTGTGTGAAAAATGAACTTTTGCCCAGTCACCC
TCTCGAGTTATCAGAAAAAAACTTCCAGCTCAACCAGGACAAGATGAACTTTTCCACGCTGAGGAACATC
CAGGGTCTGTTTGCTCCCCTGAAGTTACAGATGGAATTCAAGGCAGTGCAGCAGGTTCACCGTCTCCCGT
TTCTCCCGAGCTCAAACCTCTCACTGGATATTTTGAGGGGCAACGATGAGACCATTGGTTTTGAGGATAT
TCTTAATGATCCATCACAAAGTGAACTGATGGGTGAGCCCCACGTGATGGTGGAACATAAGCTGGGCTTG
CTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200906 representing NM_025624
Red=Cloning site Green=Tags(s)

MNARGLGSELKDSIPVAELSASGPFESHDLLRKGFSCVKNELLPSHPLELSEKNFQLNQDKMNFSTLRNI
QGLFAPLKLQMEFKAVQQVHRLPFLPSSNLSLDILRGNDETIGFEDILNDPSQSELMGEPHVMVEHKLGL
L

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025624
ORF Size 423 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025624.3
RefSeq Size 735 bp
RefSeq ORF 426 bp
Locus ID 66537
UniProt ID Q9CQT5
Cytogenetics 5 G3
Summary Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation (By similarity). Mediates the association of 20S preproteasome with the endoplasmic reticulum (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025624" in other vectors (4)
SKU Description Size Price
MC201555 Pomp (untagged) - Mouse proteasome maturation protein (Pomp), (10ug) 10 ug
$150.00
MR200906 Pomp (Myc-DDK-tagged) - Mouse proteasome maturation protein (Pomp) 10 ug
$289.00
MR200906L3 Lenti ORF clone of Pomp (Myc-DDK-tagged) - Mouse proteasome maturation protein (Pomp) 10 ug
$450.00
MR200906L4 Lenti ORF clone of Pomp (mGFP-tagged) - Mouse proteasome maturation protein (Pomp) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.