Leprotl1 (NM_026609) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200744
Leprotl1 (tGFP-tagged) - Mouse leptin receptor overlapping transcript-like 1 (Leprotl1)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Leprotl1
Synonyms 1110067H13Rik; 1520402O14Rik; AI854296
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200744 representing NM_026609
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCATCAAAGCTTTAATTAGTTTGTCCTTTGGAGGAGCGATCGGGCTGATGTTCCTGATGCTTG
GATGTGCCCTTCCGATATACAACCAATACTGGCCCCTCTTCGTTCTCTTCTTTTACATCCTCTCGCCCAT
CCCGTACTGCATAGCCAGAAGATTAGTGGATGACACGGACGCCATGAGCAACGCGTGTAAGGAGCTTGCC
ATATTCCTCACCACAGGCATCGTCGTCTCGGCTTTTGGACTCCCTGTTGTGTTTGCCAGAGCACATCTGA
TCGAGTGGGGAGCTTGCGCACTTGTTCTCACGGGAAACACAGTCATCTTCGCGACTATTCTAGGCTTCTT
CCTGGTCTTTGGAAGCAACGACGACTTCAGCTGGCAGCAGTGG


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200744 representing NM_026609
Red=Cloning site Green=Tags(s)

MAGIKALISLSFGGAIGLMFLMLGCALPIYNQYWPLFVLFFYILSPIPYCIARRLVDDTDAMSNACKELA
IFLTTGIVVSAFGLPVVFARAHLIEWGACALVLTGNTVIFATILGFFLVFGSNDDFSWQQW

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_026609
ORF Size 393 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026609.2, NP_080885.1
RefSeq Size 2609 bp
RefSeq ORF 396 bp
Locus ID 68192
UniProt ID Q9CQ74
Cytogenetics 8 A4
Summary Negatively regulates growth hormone (GH) receptor cell surface expression in liver. May play a role in liver resistance to GH during periods of reduced nutrient availability.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026609" in other vectors (4)
SKU Description Size Price
MC200144 Leprotl1 (untagged) - Mouse leptin receptor overlapping transcript-like 1 (Leprotl1), (10ug) 10 ug
$150.00
MR200744 Leprotl1 (Myc-DDK-tagged) - Mouse leptin receptor overlapping transcript-like 1 (Leprotl1) 10 ug
$289.00
MR200744L3 Lenti ORF clone of Leprotl1 (Myc-DDK-tagged) - Mouse leptin receptor overlapping transcript-like 1 (Leprotl1) 10 ug
$450.00
MR200744L4 Lenti ORF clone of Leprotl1 (mGFP-tagged) - Mouse leptin receptor overlapping transcript-like 1 (Leprotl1) 10 ug
$450.00

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