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Med31 (NM_026068) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200743
Med31 (tGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) (Med31)
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ORF Plasmid:
tGFP DDK DDK
AAV Particle:
DDK DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Med31
Synonyms 3110004H13Rik; CGI-125; l11Jus15
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200743 representing NM_026068
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGCCGTCGCTATGGAGACAGATGATGCTGGAAATCGGCTTCGGTTTCAGTTGGAGTTGGAAT
TTGTGCAATGTTTAGCCAACCCAAACTACCTTAATTTTCTTGCCCAAAGAGGTTACTTCAAAGACAAAGC
TTTTGTTAATTATCTTAAGTATTTGCTTTACTGGAAAGAACCAGAATATGCCAAATATCTAAAGTACCCT
CAGTGCTTACATATGTTAGAGCTGCTCCAGTATGAGCACTTTCGCAAGGAGCTGGTGAATGCACAGTGTG
CGAAGTTCATTGACGAGCAGCAGATTCTGCACTGGCAGCACTACTCTCGGAAGCGGGTGCGTCTTCAGCA
AGCCCTGGCCGAGCAGCAGCAGCAGAATAACACAGCAGGGAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200743 representing NM_026068
Red=Cloning site Green=Tags(s)

MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKEPEYAKYLKYP
QCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRVRLQQALAEQQQQNNTAGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026068
ORF Size 393 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026068.2, NP_080344.2
RefSeq Size 1179 bp
RefSeq ORF 396 bp
Locus ID 67279
UniProt ID Q9CXU1
Cytogenetics 11 B4
Summary Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026068" in other vectors (5)
SKU Description Size Price
MC202952 Med31 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) (Med31), (10ug) 10 ug
$150.00
MR200743 Med31 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) (Med31) 10 ug
$289.00
MR200743L3 Lenti ORF clone of Med31 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) (Med31) 10 ug
$450.00
MR200743L4 Lenti ORF clone of Med31 (mGFP-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) (Med31) 10 ug
$450.00
MR200753 Med31 (Myc-DDK-tagged) - Mouse mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) (Med31) 10 ug
$289.00

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