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Dnajc19 (BC024335) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200742
Dnajc19 (tGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 19 (cDNA clone MGC:35659 IMAGE:4974988)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajc19
Synonyms 1810055D05Rik; AA959924; Tim14
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200742 representing BC024335
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCACAGTGGTAGCAGTCGGGTTGACCATTGCTGCTGCAGGATTTGCAGGCCGTTATGTTTTAC
AAGCCATGAAGCATGTGGAGCCTCAAGTAAAACAAGTTTTTCAGAGCCTACCAAAATCTGCATTCGGTGG
TGGGTACTACAGAGGTGGATTTGAACCCAAAATGACAAAACGGGAAGCAGCATTAATATTAGGTGTAAGC
CCTACTGCCAATAAAGGGAAGATCAGGGATGCTCATCGCCGGATTATGCTATTAAATCACCCAGACAAGG
GTAAGCAACTTTTATTACTATACTGGACTGTTAATGGAATAGCTAATTCTTGGTGTTATGAATGTCTGTG
TGTGAGCAGTTTCCCAGTTGTGGATCACATTGTAGTGTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200742 representing BC024335
Red=Cloning site Green=Tags(s)

MASTVVAVGLTIAAAGFAGRYVLQAMKHVEPQVKQVFQSLPKSAFGGGYYRGGFEPKMTKREAALILGVS
PTANKGKIRDAHRRIMLLNHPDKGKQLLLLYWTVNGIANSWCYECLCVSSFPVVDHIVVS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC024335
ORF Size 390 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC024335, AAH24335
RefSeq Size 1716 bp
RefSeq ORF 392 bp
Locus ID 67713
Cytogenetics 3
Summary Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity (By similarity).UniProtKB/Swiss-Prot Function
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"BC024335" in other vectors (4)
SKU Description Size Price
MC207103 Dnajc19 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 19 (cDNA clone MGC:35659 IMAGE:4974988), (10ug) 10 ug
$165.00
MR200742 Dnajc19 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 19 (cDNA clone MGC:35659 IMAGE:4974988) 10 ug
$289.00
MR200742L3 Lenti ORF clone of Dnajc19 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 19 (cDNA clone MGC:35659 IMAGE:4974988) 10 ug
$450.00
MR200742L4 Lenti ORF clone of Dnajc19 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 19 (cDNA clone MGC:35659 IMAGE:4974988) 10 ug
$450.00

Plasmid Map for MG200742

Download sequence for

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Cloning scheme for MG200742
Circular map for MG200742

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