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Pea15a (NM_011063) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200734
Pea15a (tGFP-tagged) - Mouse phosphoprotein enriched in astrocytes 15A (Pea15a), transcript variant 2
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pea15a or NM_011063"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pea15a
Synonyms Mat; Mat1; Pea; PEA-; PEA-15; Pea15; Pkcs; Pkcs15
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200734 representing NM_011063
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGTACGGAACTCTCCTTCAGGACCTGACCAACAACATCACCCTTGAAGATCTGGAACAGCTCA
AGTCAGCCTGCAAGGAGGACATCCCCAGTGAGAAGAGTGAGGAGATCACCACAGGCAGTGCCTGGTTTAG
CTTCCTGGAGAGCCACAACAAGCTGGACAAAGACAACCTCTCCTACATTGAGCACATCTTTGAGATATCT
CGCCGTCCTGACCTCCTCACTATGGTGGTTGACTACAGAACCCGTGTGCTGAAGATCTCTGAGGAGGAGG
AGCTGGACACCAAGCTAACCCGTATTCCCAGTGCCAAGAAGTACAAAGACATTATCCGGCAGCCCTCTGA
AGAAGAAATCATCAAATTGGCTCCCCCACCAAAGAAGGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200734 representing NM_011063
Red=Cloning site Green=Tags(s)

MAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNLSYIEHIFEIS
RRPDLLTMVVDYRTRVLKISEEEELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011063
ORF Size 390 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011063.3
RefSeq Size 2477 bp
RefSeq ORF 393 bp
Locus ID 18611
UniProt ID Q62048
Cytogenetics 1 79.54 cM
Summary This gene encodes an adaptor protein that functions as a negative regulator of apoptosis induced by tumor necrosis factor-alpha, tumor necrosis factor-related apoptosis-inducing ligand, and Fas, through its interaction with fas-associated protein with death domain and caspase-8. It also regulates proliferation signaling by relocating the extracellular signal-regulated protein kinases 1 and 2 to the cytosol. The protein encoded by this gene contains an N-terminal death effector domain and a long, flexible C-terminal tail. In humans, the encoded protein is an endogenous substrate for protein kinase C. This protein is overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2016
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Documents
Resources
"NM_011063" in other vectors (3)
SKU Description Size Price
MR200734 Pea15a (Myc-DDK-tagged) - Mouse phosphoprotein enriched in astrocytes 15A (Pea15a), transcript variant 2 10 ug
$289.00
MR200734L3 Lenti ORF clone of Pea15a (Myc-DDK-tagged) - Mouse phosphoprotein enriched in astrocytes 15A (Pea15a), transcript variant 2 10 ug
$450.00
MR200734L4 Lenti ORF clone of Pea15a (mGFP-tagged) - Mouse phosphoprotein enriched in astrocytes 15A (Pea15a), transcript variant 2 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.