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Ensa (NM_001026212) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200510
Ensa (tGFP-tagged) - Mouse endosulfine alpha (Ensa), transcript variant 2
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ensa
Synonyms 1700020C18Rik; 2610007F17Rik; AI451924
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200510 representing NM_001026212
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCAGAAACAAGAAGAAGAAAACCCTGCGGAGGAGACCGGCGAGGAGAAGCAGGATACACAGGAGA
AAGAAGGGATTCTCCCTGAGAAAGCTGAGGAGGCAAAGCTAAAGGCCAAATATCCAAGCCTAGGACAAAA
GCCTGGAGGCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGCAAAAGTACTTTGACTCAGGAGACTAC
AACATGGCCAAAGCCAAGATGAAGAACAAGCAGCTGCCAAGTGCAGGAGCAGACAAGAACCTGGTGACCG
GTGACCACATCCCCACCCCACAGGACCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGG
G

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200510 representing NM_001026212
Red=Cloning site Green=Tags(s)

MSQKQEEENPAEETGEEKQDTQEKEGILPEKAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDY
NMAKAKMKNKQLPSAGADKNLVTGDHIPTPQDLPQRKSSLVTSKLAG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001026212
ORF Size 351 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001026212.1, NP_001021383.1
RefSeq Size 2276 bp
RefSeq ORF 354 bp
Locus ID 56205
UniProt ID P60840
Cytogenetics 3 F2.1
Summary Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001026212" in other vectors (4)
SKU Description Size Price
MC207516 Ensa (untagged) - Mouse endosulfine alpha (Ensa), transcript variant 2, (10ug) 10 ug
$165.00
MR200510 Ensa (Myc-DDK-tagged) - Mouse endosulfine alpha (Ensa), transcript variant 2 10 ug
$289.00
MR200510L3 Lenti ORF clone of Ensa (Myc-DDK-tagged) - Mouse endosulfine alpha (Ensa), transcript variant 2 10 ug
$450.00
MR200510L4 Lenti ORF clone of Ensa (mGFP-tagged) - Mouse endosulfine alpha (Ensa), transcript variant 2 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.