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Gtf2a2 (BC048705) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG200471
Gtf2a2 (tGFP-tagged) - Mouse general transcription factor II A, 2 (cDNA clone MGC:59444 IMAGE:6505667)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gtf2a2
Synonyms 12kDa, TfIIg
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200471 representing BC048705
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTATCAGTTATACAGAAATACAACTTTGGGGAACAGTCTTCAAGAGAGCCTTGATGAGCTCATAC
AGTCTCAACAGATCACCCCCCAGCTTGCGCTTCAAGTTCTACTTCAGTTTGATAAAGCTATAAATTCAGC
ATTGGCTCAGAGAGTCAGGAACAGAGTCAATTTCAGGGTAAGGATGTCTTCCTGCATCTGGAAGTCTGTA
CTGCTAACTCTGAGAGCGTACGTGGACTTAACTCACCATAGCAAGATTTGGTTAGTCTGGTTTGGGAACT
TGTTCTATAATCGTTGTGCTAAGTTCATCCATTCCATGTGTTTCAGAATTGCTTTTAGATTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200471 representing BC048705
Red=Cloning site Green=Tags(s)

MAYQLYRNTTLGNSLQESLDELIQSQQITPQLALQVLLQFDKAINSALAQRVRNRVNFRVRMSSCIWKSV
LLTLRAYVDLTHHSKIWLVWFGNLFYNRCAKFIHSMCFRIAFRF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC048705
ORF Size 342 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC048705, AAH48705
RefSeq Size 1124 bp
RefSeq ORF 344 bp
Locus ID 235459
Cytogenetics 9 D
Summary TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC048705" in other vectors (4)
SKU Description Size Price
MC207177 Gtf2a2 (untagged) - Mouse general transcription factor II A, 2 (cDNA clone MGC:59444 IMAGE:6505667), (10ug) 10 ug
$165.00
MR200471 Gtf2a2 (Myc-DDK-tagged) - Mouse general transcription factor II A, 2 (cDNA clone MGC:59444 IMAGE:6505667) 10 ug
$289.00
MR200471L3 Lenti ORF clone of Gtf2a2 (Myc-DDK-tagged) - Mouse general transcription factor II A, 2 (cDNA clone MGC:59444 IMAGE:6505667) 10 ug
$450.00
MR200471L4 Lenti ORF clone of Gtf2a2 (mGFP-tagged) - Mouse general transcription factor II A, 2 (cDNA clone MGC:59444 IMAGE:6505667) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.