Crb3 (NM_177638) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200450
Crb3 (tGFP-tagged) - Mouse crumbs homolog 3 (Drosophila) (Crb3)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Crb3
Synonyms 5730439B18; AU015179; AU041752
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200450 representing NM_177638
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCCCAGGCCTGGGGGTGCTCTTGGCGTTTGGTTTGCCGATGCTGCCCTCCGGCTGGAGCCTAA
CAGCACCGGACCCTTTCACAAATAGCACAACTCAACCCCCAGGTGATGAGTCCAACGGGGGCCTGTCTTC
AGGGGCTATTGTAGCCATCACTGTGGTCTTTTCCATTCTGGGAGTCCTCCTTATAGCAGTGGGGCTGTTT
CTGCTCATGCGAAAACTTCGGGAAAAGCGGCAGACAGAGGGCACCTACCGGCCCAGCAGTGAGGAGCAGG
TGGGTGCCCGTGCTCCACCACCCCCCAACCTCAAGCTGCCCCCGGAGGAGCGGCTCATC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200450 representing NM_177638
Red=Cloning site Green=Tags(s)

MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLF
LLMRKLREKRQTEGTYRPSSEEQVGARAPPPPNLKLPPEERLI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177638
ORF Size 339 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177638.3
RefSeq Size 1160 bp
RefSeq ORF 342 bp
Locus ID 224912
UniProt ID Q8QZT4
Cytogenetics 17 D
Summary Involved in the establishment of cell polarity in mammalian epithelial cells. Regulates the morphogenesis of tight junctions (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177638" in other vectors (4)
SKU Description Size Price
MC204193 Crb3 (untagged) - Mouse crumbs homolog 3 (Drosophila) (Crb3), (10ug) 10 ug
$150.00
MR200450 Crb3 (Myc-DDK-tagged) - Mouse crumbs homolog 3 (Drosophila) (Crb3) 10 ug
$289.00
MR200450L3 Lenti ORF clone of Crb3 (Myc-DDK-tagged) - Mouse crumbs homolog 3 (Drosophila) (Crb3) 10 ug
$450.00
MR200450L4 Lenti ORF clone of Crb3 (mGFP-tagged) - Mouse crumbs homolog 3 (Drosophila) (Crb3) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.