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Pyurf (NM_025574) Mouse Tagged ORF Clone

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SKU
MG200443
Pyurf (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $350.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pyurf or NM_025574"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pyurf
Synonyms 2610022G08Rik; AI847956; Prey
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200443 representing NM_025574
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGCGCCACGTGCCGCAGGCTCGCCCCGGCGCTGCGGAGGCTCCGCGCACTGTCTGCAGTCGCCG
GGAGGTTTCTGCAAGTGCCCGGGGCCAGGCTCTGCTCTGACCAGAGCGAGAGGGCTGAGCAGCCCCACAC
CTTCCACCCGGCGCTGCTGCAGTTCCTGGTGTGTCCGCTCTCCAAGAAGCCGCTTAGATATGAAGCATCG
ACAAATGAATTGGTTAATGAAGAGTTGGGAATAGCATATCCAATCATTGATGGGATCCCTAATATGATAC
CACAGGCAGCAAGGACCACACGTCAAAATGAGAAGCAAGAAGAAGCTGAGCAACCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200443 representing NM_025574
Red=Cloning site Green=Tags(s)

MLSATCRRLAPALRRLRALSAVAGRFLQVPGARLCSDQSERAEQPHTFHPALLQFLVCPLSKKPLRYEAS
TNELVNEELGIAYPIIDGIPNMIPQAARTTRQNEKQEEAEQP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025574
ORF Size 336 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025574.3, NP_079850.1
RefSeq Size 5410 bp
RefSeq ORF 339 bp
Locus ID 66459
UniProt ID Q9D1C3
Cytogenetics 6 B3
Summary This gene encodes a small protein with a conserved DUF343 domain. The human ortholog of this gene expresses two distinct proteins from upstream and downstream coding regions. The upstream CDS encoding a DUF343 domain-containing protein has been conserved at this mouse locus, but the downstream CDS encoding a subunit of an enzyme involved in glycosylphosphatidylinositol biosynthesis has not been conserved. Instead, a separate locus on mouse chromosome 9 encodes the mouse homolog of the human phosphatidylinositol glycan anchor biosynthesis, class Y protein. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_025574" in other vectors (4)
SKU Description Size Price
MC202444 Pyurf (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$150.00
MR200443 Pyurf (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR200443L3 Lenti ORF clone of Pyurf (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200443L4 Lenti ORF clone of Pyurf (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Y (Pigy), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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