Anapc16 (NM_025514) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200416
Anapc16 (tGFP-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 641, expressed (D10Ertd641e)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Anapc16
Synonyms 2310005G07Rik; APC16; D10Ertd641e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200416 representing NM_025514
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCTCCTCGTCTTCGTCTTCAGCTGGTGGGGTCAGTGGAAGCTCTGTCACCGGATCTGGTTTCA
GTGTGTCCGACCTTGCCCCACCTCGGAAAGCTCTGTTCACCTACCCGAAAGGAGCTGGAGAGATGTTAGA
AGATGGCTCCGAGCGATTCCTCTGTGAGTCGGTGTTTAGCTATCAAGTGGCATCCACGCTTAAACAGGTG
AAGCATGATCAGCAAGTCGCTCGGATGGAAAAACTGGCTGGTCTGGTGGAAGAGCTGGAGGCCGACGAGT
GGCGGTTTAAACCCATCGAGCAGCTGCTGGGGTTCACACCCTCCTCGGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200416 representing NM_025514
Red=Cloning site Green=Tags(s)

MAASSSSSSAGGVSGSSVTGSGFSVSDLAPPRKALFTYPKGAGEMLEDGSERFLCESVFSYQVASTLKQV
KHDQQVARMEKLAGLVEELEADEWRFKPIEQLLGFTPSSG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025514
ORF Size 330 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025514.3
RefSeq Size 1329 bp
RefSeq ORF 333 bp
Locus ID 52717
UniProt ID Q9CPV2
Cytogenetics 10 29.66 cM
Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025514" in other vectors (3)
SKU Description Size Price
MR200416 Anapc16 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 16 (Anapc16) 10 ug
$289.00
MR200416L3 Lenti ORF clone of Anapc16 (Myc-DDK-tagged) - Mouse anaphase promoting complex subunit 16 (Anapc16) 10 ug
$450.00
MR200416L4 Lenti ORF clone of Anapc16 (mGFP-tagged) - Mouse anaphase promoting complex subunit 16 (Anapc16) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.