OriGene Logo

Txn1 (NM_011660) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG200355
Txn1 (tGFP-tagged) - Mouse thioredoxin 1 (Txn1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $425.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (3)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8
100 ul
$447.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Txn1 or NM_011660"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Txn1
Synonyms ADF; AW550880; Trx1; Txn
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200355 representing NM_011660
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGCTGATCGAGAGCAAGGAAGCTTTTCAGGAGGCCCTGGCCGCCGCGGGAGACAAGCTTGTCG
TGGTGGACTTCTCTGCTACGTGGTGTGGACCTTGCAAAATGATCAAGCCCTTCTTCCATTCCCTCTGTGA
CAAGTATTCCAATGTGGTGTTCCTTGAAGTGGATGTGGATGACTGCCAGGATGTTGCTGCAGACTGTGAA
GTCAAATGCATGCCGACCTTCCAGTTTTATAAAAAGGGTCAAAAGGTGGGGGAGTTCTCCGGTGCTAACA
AGGAAAAGCTTGAAGCCTCTATTACTGAATATGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200355 representing NM_011660
Red=Cloning site Green=Tags(s)

MVKLIESKEAFQEALAAAGDKLVVVDFSATWCGPCKMIKPFFHSLCDKYSNVVFLEVDVDDCQDVAADCE
VKCMPTFQFYKKGQKVGEFSGANKEKLEASITEYA

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011660
ORF Size 315 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011660.3, NP_035790.1
RefSeq Size 1051 bp
RefSeq ORF 318 bp
Locus ID 22166
UniProt ID P10639
Cytogenetics 4 31.87 cM
Summary Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (By similarity). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources
"NM_011660" in other vectors (6)
SKU Description Size Price
MC201065 Txn1 (untagged) - Mouse thioredoxin 1 (Txn1), (10ug) 10 ug
$225.00
MR200355 Txn1 (Myc-DDK-tagged) - Mouse thioredoxin 1 (Txn1) 10 ug
$225.00
MR200355L1 Lenti ORF clone of Txn1 (Myc-DDK-tagged) - Mouse thioredoxin 1 (Txn1) 10 ug
$525.00
MR200355L2 Lenti ORF clone of Txn1 (mGFP-tagged) - Mouse thioredoxin 1 (Txn1) 10 ug
$525.00
MR200355L3 Lenti ORF clone of Txn1 (Myc-DDK-tagged) - Mouse thioredoxin 1 (Txn1) 10 ug
$525.00
MR200355L4 Lenti ORF clone of Txn1 (mGFP-tagged) - Mouse thioredoxin 1 (Txn1) 10 ug
$525.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.