Ndufb3 (NM_025597) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG200352
Ndufb3 (tGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 (Ndufb3)
  $350.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndufb3
Synonyms 2700033I16Rik; CI-B12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG200352 representing NM_025597
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTGGACATGGACATGAACATGGACATGAACATGGACATGGCCATGGTAAAATGGAACTTCCAG
ATTACAGACAGTGGAAAATTGAAGGGACGCCATTAGAAACGGTGCAGAAGAAGCTTGCTGCCCGAGGGCT
GAGGGATCCATGGGCTCGCAATGAGGCTTGGAGATACATGGGCGGCTTTGCAGGCAACATCACCTTCCCG
AGTGTAATATTAAAAGGATTCAAATGGGGGTTTGCTGCGTTTGTGGTAGCTTTGGGGGCTGAATATTTCC
TGGATTCCCAGAATGGTGATAAGAAGCATCAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG200352 representing NM_025597
Red=Cloning site Green=Tags(s)

MAAGHGHEHGHEHGHGHGKMELPDYRQWKIEGTPLETVQKKLAARGLRDPWARNEAWRYMGGFAGNITFP
SVILKGFKWGFAAFVVALGAEYFLDSQNGDKKHH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025597
ORF Size 312 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025597.3
RefSeq Size 533 bp
RefSeq ORF 315 bp
Locus ID 66495
UniProt ID Q9CQZ6
Cytogenetics 1 C1.3
Summary Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025597" in other vectors (4)
SKU Description Size Price
MC203221 Ndufb3 (untagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 (Ndufb3), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$150.00
MR200352 Ndufb3 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 (Ndufb3), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR200352L3 Lenti ORF clone of Ndufb3 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 (Ndufb3), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR200352L4 Lenti ORF clone of Ndufb3 (mGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 (Ndufb3), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.